NAME

Bio::SeqUtils - Additional methods for PrimarySeq objects

SYNOPSIS

    # get a Bio::PrimarySeqI compliant object, $seq, somehow
    $util = new Bio::SeqUtils;
    $poplypeptide_3char = $util->seq3($seq);
    #or
    $poplypeptide_3char = Bio::SeqUtils->seq3($seq);

    #set the sequence string (stored in one char code in the object)
    Bio::SeqUtils->seq3($seq, $poplypeptide_3char);

DESCRIPTION

This class is a holder of methods that work on Bio::PrimarySeqI compliant sequence objects, e.g. Bio::PrimarySeq and Bio::Seq. These methods are not part of the Bio::PrimarySeqI interface and should in general not essential to the primary function of sequence objects. If you are thinking of adding essential functions, it might be better to create your own sequence class.

The methods take as their first argument a sequence object. It is possible to use methods without first creating a SeqUtils object, i.e. use it as an anonymous hash.

The first two methodsgive out or read in protein sequences coded in three letter IUPAC amino acid codes.

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated.

  bioperl-l@bioperl.org          - General discussion
  http://bio.perl.org/MailList.html             - About the mailing lists

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via email or the web:

  bioperl-bugs@bio.perl.org
  http://bio.perl.org/bioperl-bugs/

AUTHOR - Heikki Lehvaslaiho

Email: heikki@ebi.ac.uk Address:

     EMBL Outstation, European Bioinformatics Institute
     Wellcome Trust Genome Campus, Hinxton
     Cambs. CB10 1SD, United Kingdom

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

seq3

 Title   : seq3
 Usage   : $string = Bio::SeqUtils->seq3($seq)
 Function:

           Read only method that returns the amino acid sequence as a
           string of three letter codes. moltype has to be
           'protein'. Output follows the IUPAC standard plus 'Ter' for
           terminator. Any unknown character, including the default
           unknown character 'X', is changed into 'Xaa'. A noncoded
           aminoacid selenocystein is recognized (Sel, U).

 Returns : A scalar
 Args    : character used for stop in the protein seqence optional,
           defaults to '*' string used to separate the output amino
           acid codes, optional, defaults to ''

seq3in

 Title   : seq3in
 Usage   : $string = Bio::SeqUtils->seq3in($seq, 'MetGlyTer')
 Function:

           Read only method that returns the amino acid sequence as a
           string of three letter codes. moltype has to be
           'protein'. Output follows the IUPAC standard plus 'Ter' for
           terminator. Any unknown character, including the default
           unknown character 'X', is changed into 'Xaa'

 Returns : Bio::PrimarySeq object;
 Args    : character to be used for stop in the protein seqence,
              optional, defaults to '*'
           character to be used for unknown in the protein seqence,
              optional, defaults to 'X'
           string used to separate the output amino acid codes,
              optional, defaults to ''