Bio::Tools::Primer::Feature - position of a single primer
use Bio::Tools::Primer::Feature; my $pf = Bio::Tools::Primer::Feature->new( -start => $start, -end => $end, -strand => $strand); $pf->attach_seq($seq); # is a SeqFeatureI print "primer starts at ",$pf->start," with sequence ",$pf->seq->seq(),"\n"; # helper functions print "GC percentage ",$pf->gc(),"\n"; print "has inversion of size 4 at ",$pf->inversion(4),"\n";
Primer Features represents one primer in a primer pair. This object is mainly for designing primers, and probably principly used in the primer design system
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The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
To install Bio::Seq, copy and paste the appropriate command in to your terminal.
cpanm
cpanm Bio::Seq
CPAN shell
perl -MCPAN -e shell install Bio::Seq
For more information on module installation, please visit the detailed CPAN module installation guide.