search_overview -- Render a SearchIO parser report into a simple overview graphic
search_overview -i filename [-f format] [-o outputfilename] [--labels]
This script will take any Bio::SearchIO parseable report and turn it into a simple overview graphic of the report. For our purposes we are assuming BLAST and the BLAST scores when assigning colors. Output is a PNG format file.
This is not intended to be an overly customized script, rather it should probably just be either a quick and dirty look at a report or a starting point for more complicated implementations.
The color is determined by the hit score which is currently pegged to the NCBI scheme which looks like this
RED E<gt>= 200 PURPLE 80-200 GREEN 50-80 BLUE 40-50 BLACK E<lt>40
Options: -i/--input The input filename, otherwise input is assumed from STDIN -o/--output The output filename, this is optional, if you do not provide the output filename the script will create a file using the name of the query sequence and will process all the sequences in the file. If an output filename IS provided the script will only display an image for the first one. -f/--format The SearchIO format parser to use, if not provided SearchIO will guess based on the file extension. -l/--labels Display the hit sequence name as a label in the overview. For lots of sequences this will make the image very long so by default it is turned off.
Jason Stajich, jason[-at-]open-bio[-dot-]org.
To install Bio::Graphics, copy and paste the appropriate command in to your terminal.
cpanm
cpanm Bio::Graphics
CPAN shell
perl -MCPAN -e shell install Bio::Graphics
For more information on module installation, please visit the detailed CPAN module installation guide.