Bio::Polloc::Locus::amplicon - An amplification product
A locus amplifiable by PCR. Implements Bio::Polloc::LocusI.
Email lmrodriguezr at gmail dot com
Methods provided by the package
Creates a Bio::Polloc::Locus::repeat object.
A Bio::Polloc::Polloc::IO file containing the primers able to amplify the locus. The format is as expected by primersearch.
Mismatches in the amplification.
A Bio::Polloc::Locus::repeat object.
Gets/sets the mismatches in the amplification.
The error int.
The error int or undef.
undef
Sets the primers based on a Bio::Polloc::Polloc::IO object.
A Bio::Polloc::Polloc::IO object.
Gets/sets the FWD primer (str).
Gets/sets the REV primer (str).
Gets the score
The score (float or undef). As the percentage of the primers matching the target sequence.
Sets/gets the errors (in number of nucleotides).
The errors (int, optional).
The errors (int or undef).
Returns the difference in length with the given locus.
The locus to compare with.
The reference locus. If set, replaces the current loaded object.
Float, the difference in length.
Bio::Polloc::Polloc::Error if no locus or the loci are not of the proper type.
Methods intended to be used only within the scope of Bio::Polloc::*
To install Bio::Polloc, copy and paste the appropriate command in to your terminal.
cpanm
cpanm Bio::Polloc
CPAN shell
perl -MCPAN -e shell install Bio::Polloc
For more information on module installation, please visit the detailed CPAN module installation guide.