NAME

Bio::LocatableSeq - A Sequence object with start/end points on it that can be projected into a MSA or have coordinates relative to another seq.

SYNOPSIS

    use Bio::LocatableSeq;
    my $seq = new Bio::LocatableSeq(-seq => "CAGT-GGT",
                                    -id  => "seq1",
                                    -start => 1,
                                    -end   => 7);

DESCRIPTION

    # a normal sequence object
    $locseq->seq();
    $locseq->id();

    # has start,end points
    $locseq->start();
    $locseq->end();

    # inherits off RangeI, so range operations possible

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated.

  bioperl-l@bioperl.org                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

The locatable sequence object was developed mainly because the SimpleAlign object requires this functionality, and in the rewrite of the Sequence object we had to decide what to do with this.

It is, to be honest, not well integrated with the rest of bioperl, for example, the trunc() function does not return a LocatableSeq object, as some might have thought. There are all sorts of nasty gotcha's about interactions between coordinate systems when these sort of objects are used.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:

  http://bugzilla.open-bio.org/

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

start

 Title   : start
 Usage   : $obj->start($newval)
 Function:
 Returns : value of start
 Args    : newvalue (optional)

end

 Title   : end
 Usage   : $obj->end($newval)
 Function:
 Returns : value of end
 Args    : newvalue (optional)

strand

 Title   : strand
 Usage   : $obj->strand($newval)
 Function:
 Returns : value of strand
 Args    : newvalue (optional)

get_nse

 Title   : get_nse
 Usage   :
 Function: read-only name of form id/start-end
 Example :
 Returns :
 Args    :

no_gap

 Title   : no_gaps
 Usage   :$self->no_gaps('.')
 Function:

           Gets number of gaps in the sequence. The count excludes
           leading or trailing gap characters.

           Valid bioperl sequence characters are [A-Za-z\-\.\*]. Of
           these, '.' and '-' are counted as gap characters unless an
           optional argument specifies one of them.

 Returns : number of internal gaps in the sequnce.
 Args    : a gap character (optional)

column_from_residue_number

 Title   : column_from_residue_number
 Usage   : $col = $seq->column_from_residue_number($resnumber)
 Function:

           This function gives the position in the alignment
           (i.e. column number) of the given residue number in the
           sequence. For example, for the sequence

             Seq1/91-97 AC..DEF.GH

           column_from_residue_number(94) returns 5.

           An exception is thrown if the residue number would lie
           outside the length of the aligment
           (e.g. column_from_residue_number( "Seq2", 22 )

 Returns : A column number for the position of the
           given residue in the given sequence (1 = first column)
 Args    : A residue number in the whole sequence (not just that
           segment of it in the alignment)

location_from_column

 Title   : location_from_column
 Usage   : $loc = $ali->location_from_column($column_number)
 Function:

           This function gives the residue number for a given position
           in the alignment (i.e. column number) of the given. Gaps
           complicate this process and force the output to be a
           L<Bio::Range> where values can be undefined. For example,
           for the sequence:

             Seq/91-97 .AC..DEF.G.

           location_from_column( 3 ) position 93
           location_from_column( 2 ) position 92^93
           location_from_column(10 ) position 97^98
           location_from_column( 1 ) position undef

           An exact position returns a Bio::Location::Simple object
           where where location_type() returns 'EXACT', if a position
           is between bases location_type() returns 'IN-BETWEEN'.
           Column before the first residue returns undef. Note that if
           the position is after the last residue in the alignment,
           that there is no guarantee that the original sequence has
           residues after that position.

           An exception is thrown if the column number is not within
           the sequence.

 Returns : Bio::Location::Simple or undef
 Args    : A column number
 Throws  : If column is not within the sequence

See Bio::Location::Simple for more.

revcom

 Title   : revcom
 Usage   : $rev = $seq->revcom()
 Function: Produces a new Bio::LocatableSeq object which
           has the reversed complement of the sequence. For protein
           sequences this throws an exception of "Sequence is a
           protein. Cannot revcom"

 Returns : A new Bio::LocatableSeq object
 Args    : none

trunc

 Title   : trunc
 Usage   : $subseq = $myseq->trunc(10,100);
 Function: Provides a truncation of a sequence,

 Example :
 Returns : a fresh Bio::PrimarySeqI implementing object
 Args    : Two integers denoting first and last columns of the
           sequence to be included into sub-sequence.

cpanm Bio::Seq

perl -MCPAN -e shell
install Bio::Seq