use strict;
use Exporter 'import';
use vars qw/ @EXPORT_OK /;
use Encode qw( decode_utf8 );
use File::chdir;
use Graph;
use IPC::Run qw/ run binary /;
@EXPORT_OK = qw/ character_input phylip_pars parse_newick newick_to_svg /;
=head1 NAME
Text::Tradition::StemmaUtil - standalone utilities for distance tree calculations
=head1 DESCRIPTION
This package contains a set of utilities for running phylogenetic analysis on
text collations.
=head1 SUBROUTINES
=head2 character_input( $tradition, $opts )
Returns a character matrix string suitable for Phylip programs, which
corresponds to the given alignment table. See Text::Tradition::Collation
for a description of the alignment table format. Options include:
=over
=item * exclude_layer - Exclude layered witnesses from the character input,
using only the 'main' text of the witnesses in the tradition.
=item * collapse - A reference to an array of relationship names that should
be treated as equivalent for the purposes of generating the character matrix.
=back
=cut
sub character_input {
my ( $tradition, $opts ) = @_;
my $table = $tradition->collation->alignment_table;
if( $opts->{exclude_layer} ) {
# Filter out all alignment table rows that do not correspond
# to a named witness - these are the layered witnesses.
my $newtable = { alignment => [], length => $table->{length} };
foreach my $row ( @{$table->{alignment}} ) {
if( $tradition->has_witness( $row->{witness} ) ) {
push( @{$newtable->{alignment}}, $row );
}
}
$table = $newtable;
}
my $character_matrix = _make_character_matrix( $table, $opts );
my $input = '';
my $rows = scalar @{$character_matrix};
my $columns = scalar @{$character_matrix->[0]} - 1;
$input .= "\t$rows\t$columns\n";
foreach my $row ( @{$character_matrix} ) {
$input .= join( '', @$row ) . "\n";
}
return $input;
}
sub _make_character_matrix {
my( $table, $opts ) = @_;
# Push the names of the witnesses to initialize the rows of the matrix.
my @matrix = map { [ _normalize_witname( $_->{'witness'} ) ] }
@{$table->{'alignment'}};
foreach my $token_index ( 0 .. $table->{'length'} - 1) {
my @pos_tokens = map { $_->{'tokens'}->[$token_index] }
@{$table->{'alignment'}};
my @pos_readings = map { $_ ? $_->{'t'} : $_ } @pos_tokens;
my @chars = _convert_characters( \@pos_readings, $opts );
foreach my $idx ( 0 .. $#matrix ) {
push( @{$matrix[$idx]}, $chars[$idx] );
}
}
return \@matrix;
}
# Helper function to make the witness name something legal for pars
sub _normalize_witname {
my( $witname ) = @_;
$witname =~ s/\s+/ /g;
$witname =~ s/[\[\]\(\)\:;,]//g;
$witname = substr( $witname, 0, 10 );
return sprintf( "%-10s", $witname );
}
sub _convert_characters {
my( $row, $opts ) = @_;
# This is a simple algorithm that treats every reading as different.
# Eventually we will want to be able to specify how relationships
# affect the character matrix.
my %unique = ( '__UNDEF__' => 'X',
'#LACUNA#' => '?',
);
my %equivalent;
my %count;
my $ctr = 0;
foreach my $rdg ( @$row ) {
next unless $rdg;
next if $rdg->is_lacuna;
next if exists $unique{$rdg->text};
if( ref( $opts->{'collapse'} ) eq 'ARRAY' ) {
my @exclude_types = @{$opts->{'collapse'}};
my @set = $rdg->related_readings( sub { my $rel = shift;
$rel->colocated && grep { $rel->type eq $_ } @exclude_types } );
push( @set, $rdg );
my $char = chr( 65 + $ctr++ );
map { $unique{$_->text} = $char } @set;
$count{$rdg->text} += scalar @set;
} else {
$unique{$rdg->text} = chr( 65 + $ctr++ );
$count{$rdg->text}++;
}
}
# Try to keep variants under 8 by lacunizing any singletons.
if( scalar( keys %unique ) > 8 ) {
foreach my $word ( keys %count ) {
if( $count{$word} == 1 ) {
$unique{$word} = '?';
}
}
}
my %u = reverse %unique;
if( scalar( keys %u ) > 8 ) {
warn "Have more than 8 variants on this location; phylip will break";
}
my @chars = map { $_ ? $unique{$_->text} : $unique{'__UNDEF__' } } @$row;
return @chars;
}
=head2 phylip_pars( $character_matrix )
Runs Phylip Pars on the given character matrix. Returns results in Newick format.
=cut
sub phylip_pars {
my( $charmatrix ) = @_;
# Set up a temporary directory for all the default Phylip files.
my $phylip_dir = File::Temp->newdir();
# $phylip_dir->unlink_on_destroy(0);
# We need an infile, and we need a command input file.
open( MATRIX, ">$phylip_dir/infile" ) or die "Could not write $phylip_dir/infile";
print MATRIX $charmatrix;
close MATRIX;
open( CMD, ">$phylip_dir/cmdfile" ) or die "Could not write $phylip_dir/cmdfile";
## TODO any configuration parameters we want to set here
# U Search for best tree? Yes
# S Search option? More thorough search
# V Number of trees to save? 100
# J Randomize input order of species? No. Use input order
# O Outgroup root? No, use as outgroup species 1
# T Use Threshold parsimony? No, use ordinary parsimony
# W Sites weighted? No
# M Analyze multiple data sets? No
# I Input species interleaved? Yes
# 0 Terminal type (IBM PC, ANSI, none)? ANSI
# 1 Print out the data at start of run No
# 2 Print indications of progress of run Yes
# 3 Print out tree Yes
# 4 Print out steps in each site No
# 5 Print character at all nodes of tree No
# 6 Write out trees onto tree file? Yes
print CMD "Y\n";
close CMD;
# And then we run the program.
my $program = File::Which::which( 'pars' );
unless( $program && -x $program ) {
throw( "Phylip pars not found in path" );
}
{
# We need to run it in our temporary directory where we have created
# all the expected files.
local $CWD = $phylip_dir;
my @cmd = ( $program );
run \@cmd, '<', 'cmdfile', '>', '/dev/null';
}
# Now our output should be in 'outfile' and our tree in 'outtree',
# both in the temp directory.
my @outtree;
if( -f "$phylip_dir/outtree" ) {
open( TREE, "$phylip_dir/outtree" ) or die "Could not open outtree for read";
@outtree = <TREE>;
close TREE;
}
return join( '', @outtree ) if @outtree;
# If we got this far, we are about to throw an error.
my @error;
if( -f "$phylip_dir/outfile" ) {
open( OUTPUT, "$phylip_dir/outfile" ) or die "Could not open output for read";
@error = <OUTPUT>;
close OUTPUT;
} else {
push( @error, "Neither outtree nor output file was produced!" );
}
throw( join( '', @error ) );
}
=head2 parse_newick( $newick_string )
Parses the given Newick tree(s) into one or more Stemma objects with
undirected graphs.
=cut
sub parse_newick {
my $newick = shift;
my @stemmata;
# Parse the result into a tree
my $forest = Bio::Phylo::IO->parse(
-format => 'newick',
-string => $newick,
);
# Turn the tree into a graph, starting with the root node
foreach my $tree ( @{$forest->get_entities} ) {
my $stemma = Text::Tradition::Stemma->new(
graph => _graph_from_bio( $tree ),
is_undirected => 1 );
push( @stemmata, $stemma );
}
return \@stemmata;
}
sub _graph_from_bio {
my $tree = shift;
my $graph = Graph->new( 'undirected' => 1 );
# Give all the intermediate anonymous nodes a name.
my $i = 0;
my $classes = {};
foreach my $n ( @{$tree->get_terminals} ) {
# The terminal nodes are our named witnesses.
$classes->{$n->get_name} = 'extant';
}
foreach my $n ( @{$tree->get_internals} ) {
unless( defined $n->get_name && $n->get_name ne '' ) {
# Get an integer, make sure it's a unique name
while( exists $classes->{$i} ) {
$i++;
}
$n->set_name( $i++ );
}
$classes->{$n->get_name} = 'hypothetical';
}
_add_tree_children( $graph, $classes, undef, [ $tree->get_root ]);
return $graph;
}
sub _add_tree_children {
my( $graph, $classes, $parent, $tree_children ) = @_;
foreach my $c ( @$tree_children ) {
my $child = $c->get_name;
$graph->add_vertex( $child );
$graph->set_vertex_attribute( $child, 'class', $classes->{$child} );
$graph->add_path( $parent, $child ) if defined $parent;
_add_tree_children( $graph, $classes, $child, $c->get_children() );
}
}
=head2 newick_to_svg( $newick_string )
Uses the FigTree utility (if installed) to transform the given Newick tree(s)
into a graph visualization.
=cut
sub newick_to_svg {
my $newick = shift;
my $program = File::Which::which( 'figtree' );
unless( -x $program ) {
throw( "FigTree commandline utility not found in path" );
}
my $svg;
my $nfile = File::Temp->new();
print $nfile $newick;
close $nfile;
my @cmd = ( $program, '-graphic', 'SVG', $nfile );
run( \@cmd, ">", binary(), \$svg );
return decode_utf8( $svg );
}
sub throw {
Text::Tradition::Error->throw(
'ident' => 'StemmaUtil error',
'message' => $_[0],
);
}
1;
=head1 LICENSE
This package is free software and is provided "as is" without express
or implied warranty. You can redistribute it and/or modify it under
the same terms as Perl itself.
=head1 AUTHOR
Tara L Andrews E<lt>aurum@cpan.orgE<gt>