————————————————————————————# BioPerl module: FAST::Bio::SeqIO::agave
#
# AGAVE: Architecture for Genomic Annotation, Visualization and Exchange.
#
# You may distribute this module under the same terms as perl itself
#
# POD documentation - main docs before the code
#
# The original version of the module can be found here:
#
# ### TODO: live link for this anymore?
# The DTD for AGAVE XML was once located here (dead link):
#
#
=head1 NAME
FAST::Bio::SeqIO::agave - AGAVE sequence output stream.
=head1 SYNOPSIS
It is probably best not to use this object directly, but
rather go through the SeqIO handler system. Go:
$in = FAST::Bio::SeqIO->new('-file' => "$file_in",
'-format' => 'EMBL');
$out = FAST::Bio::SeqIO->new('-file' => ">$file_out",
'-format' => 'AGAVE');
while (my $seq = $in->next_seq){
$out->write_seq($seq);
}
=head1 DESCRIPTION
This object can transform FAST::Bio::Seq objects to agave xml file and
vice-versa. I (Simon) coded up this module because I needed a parser
to extract data from AGAVE xml to be utitlized by the GenQuire genome
annotation system (See http://www.bioinformatics.org/Genquire).
***NOTE*** At the moment, not all of the tags are implemented. In
general, I followed the output format for the XEMBL project
=cut
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution.
Bug reports can be submitted via the web:
=head1 AUTHOR - Simon K. Chan
Email:
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
# ===================
# Let the code begin...
package
FAST::Bio::SeqIO::agave;
use
strict;
use
IO::File;
use
FAST::Bio::Seq;
use
FAST::Bio::Species;
use
XML::Writer;
use
Data::Dumper;
# ==================================================================================
sub
_initialize {
my
(
$self
,
@args
) =
@_
;
$self
->SUPER::_initialize(
@args
);
# Run the constructor of the parent class.
my
%tmp
=
@args
;
$self
->{
'file'
} =
$tmp
{
'-file'
};
if
(
$self
->{
'file'
} !~ /^>/) {
$self
->_process;
# Parse the thing, but only if it is the input file (ie not
# outputing agave file, but reading it).
$self
->{
'parsed'
} = 1;
# Set the flag to let the code know that the agave xml file
# has been parsed.
}
$self
->{
'seqs_stored'
} = 0;
}
# ==================================================================================
=head2 _process
Title : _process
Usage : $self->_process
Function : Parses the agave xml file.
Args : None.
Returns : Nothing.
Note : Method(s) that call(s) this method : _initialize
Method(s) that this method calls : _process_sciobj
FIRST/START sub.
=cut
sub
_process {
my
(
$self
) =
@_
;
while
(1) {
my
$line
=
$self
->_readline;
next
unless
$line
;
next
if
$line
=~ /^\s*$/;
if
(
$line
=~ /<\?xml version/o) {
# do nothing
}
elsif
(
$line
=~ /\<!DOCTYPE (\w+) SYSTEM
"([\w\.]+)"
\>/) {
$self
->throw(
"Error: This xml file is not in AGAVE format! DOCTYPE: $1 , SYSTEM: $2\n\n"
)
if
$1 ne
'sciobj'
|| $2 ne
'sciobj.dtd'
;
}
elsif
(
$line
=~ /<sciobj (.*)>/) {
push
@{
$self
->{
'sciobj'
}},
$self
->_process_sciobj($1);
}
elsif
(
$line
=~ /<\/sciobj>/) {
last
;
# It is finished.
}
else
{
# throw an error message. The above conditions should
# take care all of the possible options...?
# $self->throw("Error: Do not recognize this AGAVE xml
# line: $line\n\n");
}
}
# close while loop
return
;
}
# ==================================================================================
=head2 _process_sciobj
Title : _process_sciobj
Usage : $self->_process_sciobj
Function : Parses the data between the <sciobj></sciobj> tags.
Args : The string that holds the attributes for <sciobj>.
Returns : Data structure holding the values parsed between
the <sciobj></sciobj> tags.
Note : Method(s) that call(s) this method : _process
Method(s) that this method calls :
_helper_store_attribute_list , _process_contig
=cut
sub
_process_sciobj {
my
(
$self
,
$attribute_line
) =
@_
;
my
$sciobj
;
$self
->_helper_store_attribute_list(
$attribute_line
, \
$sciobj
);
my
$line
=
$self
->_readline;
# Zero or more <contig>
while
(
$line
=~ /<contig\s?(.*?)\s?>/) {
my
$contig
=
$self
->_process_contig(\
$line
, $1);
push
@{
$sciobj
->{
'contig'
}},
$contig
;
# print "line in _process_sciobj: $line\n";
# $line changes value within the subs called in this sub (_process_contig).
}
return
$sciobj
;
}
# ==================================================================================
=head2 _process_contig
Title : _process_contig
Usage : $self->_process_contig
Function : Parses the data between the <contig></contig> tags.
Args : 2 scalars:
- reference to a scalar holding the line to be parsed.
- scalar holding the attributes for the <contig> tag
to be parsed.
Returns : Data structure holding the values parsed between
the <contig></contig> tags.
Note : Method(s) that call(s) this method : _process_sciobj
Method(s) that this method calls :
_helper_store_attribute_list, _one_tag , _process_fragment_order
=cut
sub
_process_contig {
my
(
$self
,
$line
,
$attribute_line
) =
@_
;
my
$contig
;
$self
->_helper_store_attribute_list(
$attribute_line
, \
$contig
);
$$line
=
$self
->_readline;
# One <db_id>:
$self
->_one_tag(
$line
, \
$contig
,
'db_id'
);
# Zero or more <fragment_order>
$self
->_process_fragment_order(
$line
, \
$contig
);
return
$contig
;
}
# ==================================================================================
=head2 _process_fragment_order
Title : _process_fragment_order
Usage : $self->_process_fragment_order
Function : Parses the data between the <fragment_order></fragment_order> tags.
Args : 2 scalars:
- reference to a scalar holding the value of the line to be parsed.
- reference to a data structure to store the <fragment_order> data.
Returns : Nothing.
Note : Method(s) that call(s) this method : _process_contig
Method(s) that this method calls :
_helper_store_attribute_list , _process_fragment_orientation
=cut
sub
_process_fragment_order {
my
(
$self
,
$line
,
$data_structure
) =
@_
;
# Because I'm passing a reference to a data structure, I don't need to return it
# after values have been added.
while
(
$$line
=~ /<fragment_order\s?(.*?)\s?>/) {
my
$fragment_order
;
$self
->_helper_store_attribute_list($1, \
$fragment_order
);
# Store the attribute(s) for <fragment_order> into the
# $fragment_order data structure.
$$line
=
$self
->_readline;
# One or more <fragment_orientation>
$self
->_process_fragment_orientation(
$line
, \
$fragment_order
);
# Don't forget: $line is a reference to a scalar.
push
@{
$$data_structure
->{
'fragment_order'
}},
$fragment_order
;
# Store the data between <fragment_order></fragment_order>
# in $$data_structure.
}
return
;
}
# ==================================================================================
=head2 _process_fragment_orientation
Title : _process_fragment_orientation
Usage : $self->_process_fragment_orientation
Function : Parses the data between the <fragment_orientation> and
</fragment_orientation> tags.
Args : 2 scalars:
- reference to a scalar holding the value of the line to be parsed.
- reference to a data structure to store the <fragment_orientation> data.
Returns : Nothing.
Note : Method(s) that call(s) this method : _process_fragment_order
Method(s) that this method calls : _helper_store_attribute_list ,
_process_bio_sequence
=cut
sub
_process_fragment_orientation {
my
(
$self
,
$line
,
$data_structure
) =
@_
;
# counter to determine the number of iterations within this while loop.
my
$count
= 0;
# One or more <fragment_orientation>
while
(
$$line
=~ /<fragment_orientation\s?(.*?)\s?>/) {
my
$fragment_orientation
;
$self
->_helper_store_attribute_list($1, \
$fragment_orientation
);
$$line
=
$self
->_readline;
# One <bio_sequence>
$$line
=~ /<bio_sequence\s?(.*?)\s?>/;
# Process the data between <bio_sequence></bio_sequence>
my
$bio_sequence
=
$self
->_process_bio_sequence(
$line
, $1);
$fragment_orientation
->{
'bio_sequence'
} =
$bio_sequence
;
push
@{
$$data_structure
->{
'fragment_orientation'
}},
$fragment_orientation
;
++
$count
;
}
$self
->throw(
"Error: Missing <fragment_orientation> tag. Got this: $$line\n\n"
)
if
$count
== 0;
return
;
}
# ==================================================================================
=head2 _process_bio_sequence
Title : _process_bio_sequence
Usage : $self->_process_bio_sequence
Function : Parses the data between the <bio_sequence></bio_sequence> tags.
Args : 2 scalars:
- reference to a scalar holding the value of the line to be parsed.
- scalar holding the value of the attributes for <bio_sequence>
Returns : data structure holding the values between <bio_sequence></bio_sequence>
Note : Method(s) that call(s) this method : _process_fragment_orientation
Method(s) that this method calls : _helper_store_attribute_list ,
_one_tag , _question_mark_tag , _star_tag , _process_alt_ids ,
_process_xrefs , _process_sequence_map
=cut
sub
_process_bio_sequence {
my
(
$self
,
$line
,
$attribute_line
) =
@_
;
my
$bio_sequence
;
$self
->_helper_store_attribute_list(
$attribute_line
, \
$bio_sequence
);
$$line
=
$self
->_readline;
# One <db_id>.
$self
->_one_tag(
$line
, \
$bio_sequence
,
'db_id'
);
# Zero or one <note>.
$self
->_question_mark_tag(
$line
, \
$bio_sequence
,
'note'
);
# Zero or more <description>
$self
->_question_mark_tag(
$line
, \
$bio_sequence
,
'description'
);
# Zero or more <keyword>
$self
->_star_tag(
$line
, \
$bio_sequence
,
'keyword'
);
# Zero or one <sequence>
$self
->_question_mark_tag(
$line
, \
$bio_sequence
,
'sequence'
);
# Zero or one <alt_ids>
# NOT IMPLEMENTED!!!!
#if ($line =~ /<alt_ids>/){ # NOT DONE YET!
# my $alt_ids;
# $bio_sequence->{'alt_ids'} = $self->_process_alt_ids(\$alt_ids);
#}
# Zero or one <xrefs>
if
(
$$line
=~ /<xrefs\s?(.*?)\s?>/) {
my
$xrefs
=
$self
->_process_xrefs(
$line
, \
$bio_sequence
);
$bio_sequence
->{
'xrefs'
} =
$xrefs
||
'null'
;
}
# Zero or more <sequence_map>
if
(
$$line
=~ /<sequence_map\s?(.*?)\s?>/) {
my
$sequence_map
=
$self
->_process_sequence_map(
$line
);
push
@{
$bio_sequence
->{
'sequence_map'
}},
$sequence_map
;
}
# print Data::Dumper->Dump([$bio_sequence]); exit;
return
$bio_sequence
;
}
# ==================================================================================
=head2 _process_xrefs
Title : _process_xrefs
Usage : $self->_process_xrefs
Function : Parse the data between the <xrefs></xrefs> tags.
Args : reference to a scalar holding the value of the line to be parsed.
Return : Nothing.
Note : Method(s) that call(s) this method: _process_bio_sequence
Method(s) that this method calls: _one_tag , _process_xref
=cut
sub
_process_xrefs {
my
(
$self
,
$line
) =
@_
;
my
$xrefs
;
$$line
=
$self
->_readline;
# One or more <db_id> or <xref> within <xrefs></xrefs>. Check if
# to see if there's at least one.
if
(
$$line
=~ /<db_id|xref\s?(.*?)\s?>/) {
while
(
$$line
=~ /<(db_id|xref)\s?(.*?)\s?>/) {
if
($1 eq
"db_id"
) {
my
$db_id
;
$self
->_one_tag(
$line
, \
$db_id
,
'db_id'
);
push
@{
$xrefs
->{
'db_id'
}},
$db_id
;
}
elsif
($1 eq
"xref"
) {
my
$xref
;
$self
->_process_xref(
$line
, \
$xref
);
push
@{
$xrefs
->{
'xref'
}},
$xref
;
}
else
{
$self
->throw(
"Error: Tag type should be one of db_id or xref! Got this: $$line\n\n"
);
}
}
# close while loop
if
(
$$line
=~ /<\/xrefs>/) {
$$line
=
$self
->_readline;
# get the next line to be _processed by the next sub.
return
$xrefs
;
}
else
{
$self
->throw(
"Error: Missing </xrefs> tag. Got this: $$line\n\n"
);
}
}
else
{
$self
->throw(
"Error: Missing <db_id> or <xref> tag. Got this: $$line\n\n"
);
}
return
;
}
# ==================================================================================
=head2 _process_xref
Title : _process_xref
Usage : $self->_process_xref
Function : Parses the data between the <xref></xref> tags.
Args : 2 scalars:
- reference to a scalar holding the value of the line to be parsed.
- reference to a data structure to store the <xref> data.
Returns : Nothing.
Note : Method(s) that call(s) this method : _process_xrefs (note the 's' in 'xrefs')
Method(s) that this method calls : _helper_store_attribute_list , _star_tag
=cut
sub
_process_xref {
my
(
$self
,
$line
,
$xref
) =
@_
;
$$line
=
$self
->_readline;
# One <db_id>
if
(
$$line
=~ /<db_id\s?(.*?)\s?>/) {
$self
->_helper_store_attribute_list($1,
$xref
);
}
else
{
$self
->throw(
"Error: Missing <db_id> tag. Got this: $$line\n\n"
);
}
# Zero or more <xref_property>
$self
->_star_tag(
$line
,
$xref
,
'xref_propery'
);
return
;
}
# ==================================================================================
=head2 _process_sequence_map
Title : _process_sequence_map
Usage : $self->_process_sequence_map
Function : Parses the data between the <sequence_map></sequence_map> tags.
Args : Reference to scalar holding the line to be parsed.
Returns : Data structure that holds the values that were parsed.
Note : Method(s) that call(s) this method : _process_bio_sequence
Method(s) that this method calls : _helper_store_attribute_list ,
_question_mark_tag , _process_annotations
=cut
sub
_process_sequence_map {
my
(
$self
,
$line
) =
@_
;
my
$sequence_map
;
# Zero or more <sequence_map>
while
(
$$line
=~ /<sequence_map\s?(.*?)\s?>/) {
$self
->_helper_store_attribute_list($1, \
$sequence_map
)
if
defined
$1;
$$line
=
$self
->_readline;
# Zero or one <note>
$self
->_question_mark_tag(
$line
, \
$sequence_map
,
'note'
);
# NOT IMPLEMENTED!!!
#if ($$line =~ /<computations\?(.*?)\s?>/){
# # $self->_process_computations();
#}
# Zero or one <annotations>
if
(
$$line
=~ /<annotations\s?(.*?)\s?>/) {
my
$annotations
=
$self
->_process_annotations(
$line
);
$sequence_map
->{
'annotations'
} =
$annotations
;
}
}
# closes the while loop
# Match closing tag:
if
(
$$line
=~ /<\/sequence_map>/) {
return
$sequence_map
;
}
else
{
$self
->throw(
"Error: Missing </sequence_map> tag. Got this: $$line\n\n"
);
}
}
# ==================================================================================
=head2 _process_annotations
Title : _process_annotations
Usage : $self->_process_annotations
Function : Parse the data between the <annotations></annotations> tags.
Args : Reference to scalar holding the line to be parsed.
Returns : Data structure that holds the values that were parsed.
Note : Method(s) that call(s) this method : _process_sequence_map
Method(s) that this method calls : _process_seq_feature
=cut
sub
_process_annotations {
my
(
$self
,
$line
) =
@_
;
# ( seq_feature | gene | comp_result )+
my
$annotations
;
$$line
=
$self
->_readline;
my
$count
= 0;
# counter to keep track of number of iterations in the loop.
# One or more of these:
while
(
$$line
=~ /<(seq_feature|gene|comp_result)\s?(.*?)\s?>/) {
if
(
$$line
=~ /<seq_feature\s?(.*?)\s?>/) {
my
$seq_feature
=
$self
->_process_seq_feature(
$line
, $1);
push
@{
$annotations
->{
'seq_feature'
}},
$seq_feature
;
}
elsif
(
$$line
=~ /<gene\s?(.*?)\s?>/) {
# gene
}
elsif
(
$$line
=~ /<comp_result\s?(.*?)\s?>/) {
# comp_result
}
++
$count
;
}
# closes the while loop.
$self
->throw(
"Error: Missing <seq_feature> tag. Got: $$line\n\n"
)
if
$count
== 0;
# Match closing tag:
if
(
$$line
=~ /<\/annotations/) {
$$line
=
$self
->_readline;
# get the next line to be _processed by the next sub.
return
$annotations
;
}
else
{
$self
->throw(
"Error: Missing </annotations> tag. Got this: $$line\n\n"
);
}
}
# ==================================================================================
=head2 _process_seq_feature
Title : _process_seq_feature
Usage : $self->_process_seq_feature
Function : Parses the data between the <seq_feature></seq_feature> tag.
Args : 2 scalars:
- Reference to scalar holding the line to be parsed.
- Scalar holding the attributes for <seq_feature>.
Returns : Data structure holding the values parsed.
Note : Method(s) that call(s) this method: _process_annotations
Method(s) that this method calls: _helper_store_attribute_list ,
_process_classification , _question_mark_tag , _one_tag ,
_process_evidence , _process_qualifier , _process_seq_feature ,
_process_related_annot
=cut
sub
_process_seq_feature {
my
(
$self
,
$line
,
$attribute_line
) =
@_
;
my
$seq_feature
;
$self
->_helper_store_attribute_list(
$attribute_line
, \
$seq_feature
);
$$line
=
$self
->_readline;
# Zero or more <classification>
$self
->_process_classification(
$line
, \
$seq_feature
);
# Zero or one <note>
$self
->_question_mark_tag(
$line
, \
$seq_feature
,
'note'
);
# One <seq_location>
$self
->_one_tag(
$line
, \
$seq_feature
,
'seq_location'
);
# Zero or one <xrefs>
$self
->_question_mark_tag(
$line
, \
$seq_feature
,
'xrefs'
);
# Zero or one <evidence>
$self
->_process_evidence(
$line
, \
$seq_feature
);
# Zero or more <qualifier>
$self
->_process_qualifier(
$line
, \
$seq_feature
);
# Zero or more <seq_feature>. A <seq_feature> tag within a <seq_feature> tag? Oh, well. Whatever...
while
(
$$line
=~ /<seq_feature\s?(.*?)\s?>/) {
$self
->_process_seq_feature(
$line
, $1);
$$line
=
$self
->_readline;
}
# Zero or more <related_annot>
while
(
$$line
=~ /<related_annot\s?(.*?)\s?>/) {
$self
->_process_related_annot(
$line
, $1);
$$line
=
$self
->_readline;
}
# Match the closing tag:
if
(
$$line
=~ /<\/seq_feature>/) {
$$line
=
$self
->_readline;
# for the next sub...
return
$seq_feature
;
}
else
{
$self
->throw(
"Error. Missing </seq_feature> tag. Got this: $$line\n"
);
}
}
# ==================================================================================
=head2 _process_qualifier
Title : _process_qualifier
Usage : $self->_process_qualifier
Function : Parse the data between the <qualifier></qualifier> tags.
Args : 2 scalars:
- reference to a scalar holding the value of the line to be parsed.
- reference to a data structure to store the <qualifer> data.
Returns : Nothing.
Note : Method(s) that call(s) this method : _process_seq_feature
Method(s) that this method calls : _star_tag
=cut
sub
_process_qualifier {
my
(
$self
,
$line
,
$data_structure
) =
@_
;
my
$qualifier
;
$self
->_star_tag(
$line
, \
$qualifier
,
'qualifier'
);
push
@{
$$data_structure
->{
'qualifier'
}},
$qualifier
;
return
;
# No need to return the data structure since its reference was what was modified.
}
# ==================================================================================
=head2 _process_classification
Title : _process_classification
Usage : $self->_process_classification
Function: Parse the data between the <classification></classification> tags.
Args : 2 scalars:
- reference to a scalar holding the value of the line to be parsed.
- reference to a data structure to store the <qualifer> data.
Returns : Nothing.
Note : Method(s) that call(s) this method: _process_seq_feature
Method(s) that this method calls: _helper_store_attribute_list ,
_question_mark_tag , _star_tag, _process_evidence
=cut
sub
_process_classification {
# NOT IN USE.
my
(
$self
,
$line
,
$data_structure
) =
@_
;
my
$classification
=
$$data_structure
->{
'classification'
};
while
(
$$line
=~ /<classification\s?(.*?)\s?>/) {
$self
->_helper_store_attribute_list($1, \
$classification
);
# Zero or one <description>
$self
->_question_mark_tag(
$line
, \
$classification
,
'description'
);
# Zero or more <id_alias>
$self
->_star_tag(
$line
, \
$classification
,
'id_alias'
);
# Zero or one <evidence>
$self
->_process_evidence(
$line
, \
$classification
);
}
}
# ==================================================================================
sub
_process_evidence {
# NOT done.
my
(
$self
,
$line
,
$data_structure
) =
@_
;
if
(
$$line
=~ /<evidence>/) {
$$line
=
$self
->_readline;
# One or more <element_id> OR One or more <comp_result>
while
(
$$line
=~ /<(element_id|comp_result)\s?(.*?)\s?>/) {
if
(
$$line
=~ /<element_id\s?(.*?)\s?>/) {
my
$element_id
;
$self
->_plus_tag(
$line
, \
$element_id
,
'element_id'
);
push
@{
$$data_structure
->{
'element_id'
}},
$element_id
;
}
elsif
(
$$line
=~ /<comp_result\s?(.*?)\s?>/) {
my
$comp_result
;
$self
->_process_comp_result(
$line
, \
$comp_result
, $1);
push
@{
$$data_structure
->{
'comp_result'
}},
$comp_result
;
}
$$line
=
$self
->_readline;
}
}
}
# ==================================================================================
sub
_process_comp_result {
# NOT IN USE.
my
(
$self
,
$line
,
$comp_result
,
$attribute_line
) =
@_
;
$self
->_helper_store_attribute_list(
$attribute_line
,
$comp_result
);
$$line
=
$self
->_readline;
# Zero or one <note>
$self
->_question_mark_tag(
$line
,
$comp_result
,
'note'
);
# Zero or one <match_desc>
$self
->_question_mark_tag(
$line
,
$comp_result
,
'match_desc'
);
# Zero or one <match_align>
$self
->_question_mark_tag(
$line
,
$comp_result
,
'match_align'
);
# Zero or one <query_region>
$self
->_process_query_region(
$line
,
$comp_result
);
# Zero or one <match_region>
$self
->_process_match_region(
$line
,
$comp_result
);
# Zero or more <result_property>
$self
->_star_tag(
$line
,
$comp_result
,
'result_property'
);
# Zero or more <result_group>
$self
->_process_result_group(
$line
,
$comp_result
);
# Zero or more <related_annot>
$self
->_process_related_annot(
$line
,
$comp_result
);
}
# ==================================================================================
sub
_process_related_annot {
# NOT IN USE.
my
(
$self
,
$line
,
$data_structure
) =
@_
;
while
(
$$line
=~ /<related_annot\s?(.*?)\s?>/) {
my
$related_annot
;
# Zero or one <related_annot>
$self
->_helper_store_attribute_list($1, \
$related_annot
);
$$line
=
$self
->_readline;
# One or more <element_id>
my
$element_id_count
= 0;
while
(
$$line
=~ /<element_id\s?(.*?)\s?>/) {
my
$element_id
;
$self
->_helper_store_attribute_list($1, \
$element_id
);
push
@{
$related_annot
->{
'element_id'
}},
$element_id
;
$$line
=
$self
->_readline;
++
$element_id_count
;
}
if
(
$element_id_count
== 0) {
$self
->throw(
"Error. Missing <element_id> tag. Got: $$line"
);
}
# Zero or more <sci_property>
$self
->_star_tag(
$line
, \
$related_annot
,
'sci_property'
);
# while ($$line =~ /<sci_property\s?(.*?)\s?>/){
#
# }
push
@{
$data_structure
->{
'related_annot'
}},
$related_annot
;
unless
(
$$line
=~ /<\/related_annot>/){
$self
->throw(
"Error. Missing </related_tag>. Got: $$line\n"
);
}
}
}
# ==================================================================================
sub
_process_result_group {
# NOT IN USE.
my
(
$self
,
$line
,
$data_structure
) =
@_
;
while
(
$$line
=~ /<result_group\s?(.*?)\s?>/) {
my
$result_group
=
$$data_structure
->{
'result_group'
};
$self
->_helper_store_attribute_list($1, \
$result_group
);
my
$count
= 0;
$$line
=
$self
->_readline;
while
(
$$line
=~ /<comp_result\s?(.*?)\s?>/) {
# one or more <comp_result>
$self
->_process_comp_result(\
$line
, \
$result_group
, $1);
$$line
=
$self
->_readline;
++
$count
;
}
$self
->throw(
"Error. No <comp_result></comp_result> tag! Got this: $$line"
)
if
$count
== 0;
# in the last iteration in the inner while loop, $line will
# have a value of the closing tag of 'result_group'
if
(
$line
=~ /<\/result_group>/) {
$$line
=
$self
->_readline;
}
else
{
$self
->throw(
"Error. No </result_tag>! Got this: $$line"
);
}
}
}
# ==================================================================================
sub
_process_match_region {
# NOT IN USE.
my
(
$self
,
$line
,
$data_structure
) =
@_
;
my
$match_region
=
$data_structure
->{
'match_region'
};
if
(
$$line
=~ /<match_region\s?(.*?)\s?>(.*?)>/) {
$self
->_helper_store_attribute_line($1, \
$match_region
);
$$line
=
$self
->_readline;
# Zero or one db_id | element_id | bio_sequence
if
(
$$line
=~ /<db_id\s?(.*?)\s?>(.*?)<\/db_id>/) {
$self
->_question_mark_tag(
$line
, \
$match_region
,
'db_id'
);
}
elsif
(
$$line
=~ /<element_id\s?(.*?)\s?>/) {
# empty...
$self
->_question_mark_tag(
$line
, \
$match_region
,
'element_id'
);
}
elsif
(
$$line
=~ /<bio_sequence\s?(.*?)\s?>/) {
$match_region
->{
'bio_sequence'
} =
$self
->_process_bio_sequence(
$line
, $1);
}
$$line
=
$self
->_readline;
if
(
$$line
=~ /<\/match_region>/o) {
$$line
=
$self
->_readline;
# get the next line to be _processed by the next sub
return
;
}
else
{
$self
->throw(
"No closing tag </match_region>! Got this: $$line\n"
);
}
}
}
# ==================================================================================
sub
_process_query_region {
# NOT IN USE.
my
(
$self
,
$line
,
$data_structure
) =
@_
;
my
$query_region
=
$data_structure
->{
'query_region'
};
if
(
$$line
=~ /<query_region\s?(.*?)\s?>/) {
$self
->_helper_store_attribute_list($1, \
$query_region
);
$$line
=
$self
->_readline;
# Zero or one <db_id>
$self
->_question_mark_tag(
$line
, \
$query_region
,
'db_id'
);
if
(
$$line
=~ /<\/query_region>/) {
$$line
=
$self
->_readline;
# get the next line to _process.
return
;
}
else
{
$self
->throw(
"No closing tag </query_region>. Got this: $$line\n"
);
}
}
}
# ==================================================================================
=head2 _tag_processing_helper
Title : _tag_processing_helper
Usage : $self->_tag_processing_helper
Function : Stores the tag value within the data structure.
Also calls _helper_store_attribute_list to store the
attributes and their values in the data structure.
Args : 5 scalars:
- Scalar holding the value of the attributes
- Reference to a data structure to store the data for <$tag_name>
- Scalar holding the tag name.
- Scalar holding the value of the tag.
- Scalar holding the value of either 'star', 'plus',
or 'question mark' which specifies what type of method
called this method.
Returns : Nothing.
Note : Method(s) that call(s) this method:
Method(s) that this method calls: _helper_store_attribute_list
=cut
sub
_tag_processing_helper {
my
(
$self
,
$attribute_list
,
$data_structure
,
$tag_name
,
$tag_value
,
$caller
) =
@_
;
# Add the attributes to the $$data_structure if they exist.
# print "tag_name: $tag_name , attribute_list: $attribute_list\n";
if
(
defined
$attribute_list
) {
$self
->_helper_store_attribute_list(
$attribute_list
,
$data_structure
);
}
if
(
$caller
eq
'star'
||
$caller
eq
'plus'
) {
push
@{
$$data_structure
->{
$tag_name
}},
$tag_value
;
# There's either zero or more tags (*) or one or more (+)
}
else
{
$$data_structure
->{
$tag_name
} =
$tag_value
||
'null'
;
# There's zero or one tag (?)
}
return
;
}
# ==================================================================================
=head2 _one_tag
Title : _one_tag
Usage : $self->_one_tag
Function : A method to store data from tags that occurs just once.
Args : 2 scalars:
- reference to a scalar holding the value of the line to be parsed.
- reference to a data structure to store the data for <$tag_name>
Returns : Nothing.
Note : Method(s) that call(s) this method : many
Method(s) that this method calls : _tag_processing_helper
=cut
sub
_one_tag {
my
(
$self
,
$line
,
$data_structure
,
$tag_name
) =
@_
;
$self
->throw(
"Error: Missing <$tag_name></$tag_name>. Got: $$line\n\n"
)
if
$$line
!~ /\<
$tag_name
/;
# check to see if $$line is in correct format.
if
(
$$line
=~ /<
$tag_name
\s?(.*?)\s?\/?>(.*?)<\/
$tag_name
>/) {
$self
->_tag_processing_helper($1,
$data_structure
,
$tag_name
, $2,
'one'
);
# $1 = attributes $data_structure = to hold the parsed values
# # $tag_name = name of the tag $2 = tag value 'one' = lets
# _tag_processing_helper know that it was called from the
# _one_tag method.
}
elsif
(
$$line
=~ /<
$tag_name
\s?(.*?)\s?\/?>/) {
$self
->_tag_processing_helper($1,
$data_structure
,
$tag_name
,
''
,
'one'
);
}
else
{
$self
->throw(
"Error: Cannot parse this line: $$line\n\n"
);
}
$$line
=
$self
->_readline;
# get the next line.
return
;
}
# ==================================================================================
=head2 _question_mark_tag
Title : _question_mark_tag
Usage : $self->_question_mark_tag
Function : Parses values from tags that occurs zero or one time. ie: tag_name?
Args : 3 scalars:
- reference to a scalar holding the value of the line to be parsed.
- reference to a data structure to store the data for <$tag_name>
- scalar holding the name of the tag.
Returns : Nothing.
Note : Method(s) that call(s) this method : many.
Method(s) that this method calls : _tag_processing_helper
=cut
sub
_question_mark_tag {
my
(
$self
,
$line
,
$data_structure
,
$tag_name
) =
@_
;
if
(
$$line
=~ /<
$tag_name
\s?(.*?)\s?>(.*?)<\/
$tag_name
>/) {
$self
->_tag_processing_helper($1,
$data_structure
,
$tag_name
, $2,
'question mark'
);
$$line
=
$self
->_readline;
}
return
;
}
# ==================================================================================
=head2 _star_tag
Title : _star_tag
Usage : $self->_star_tag
Function : Parses values from tags that occur zero or more times. ie: tag_name*
Args : 3 scalars:
- reference to a scalar holding the value of the line to be parsed.
- reference to a data structure to store the data for <$tag_name>
- scalar holding the name of the tag.
Returns : Nothing.
Note : Method(s) that call(s) this method : many.
Method(s) that this method calls : _tag_processing_helper
=cut
sub
_star_tag {
my
(
$self
,
$line
,
$data_structure
,
$tag_name
) =
@_
;
#print "tag_name: $tag_name\n";
while
(
$$line
=~ /<
$tag_name
\s?(.*?)\s?>(.*?)<\/
$tag_name
>/) {
$self
->_tag_processing_helper
($1,
$data_structure
,
$tag_name
, $2,
'star'
);
# The tag and attribute values are stored within
# $$data_structure within the _tag_processing_helper method.
$$line
=
$self
->_readline;
}
#if ($tag_name eq 'qualifier'){
# print "this one:\n";
# print Data::Dumper->Dump([$data_structure]); exit;
#}
return
;
}
# ==================================================================================
=head2 _plus_tag
Title : _plus_tag
Usage : $self->_plus_tag
Function : Handles 'plus' tags (tags that occur one or more times). tag_name+
Args : 3 scalars:
- reference to a scalar holding the value of the line to be parsed.
- reference to a data structure to store the data for <$tag_name>
- scalar holding the name of the tag.
Returns : Nothing.
Note : Method(s) that call(s) this method : many.
Method(s) that this method calls : _star_tag
=cut
sub
_plus_tag {
my
(
$self
,
$line
,
$data_structure
,
$tag_name
) =
@_
;
if
(
$$line
=~ /<
$tag_name
\s?(.*?)\s?>(.*?)<\/
$tag_name
>/) {
# Store value of the first occurence of $tag_name.
# All subsequent values, if any, will be stored in the method _star_tag.
$self
->_tag_processing_helper($1,
$data_structure
,
$tag_name
, $2,
'plus'
);
# If the flow gets within this block, we've already determined
# that there's at least one of <$tag_name> Are there more? To
# answer this, we could just treat the tag as a * tag now
# (zero or more). We've already determined that it's NOT
# zero, so how many more? Thus, call _star_tag.
$$line
=
$self
->_readline;
$self
->_star_tag(
$line
,
$data_structure
,
$tag_name
);
}
else
{
$self
->throw(
"Error: Missing <$tag_name></$tag_name>. Got: $$line\n\n"
);
}
return
;
}
# ==================================================================================
=head2 _helper_store_attribute_list
Title : _helper_store_attribute_list
Usage : $self->_helper_store_attribute_list
Function : A helper method used to store the attributes from
the tags into the data structure.
Args : 2 scalars:
- scalar holding the attribute values to be parsed.
- reference to a data structure to store the data between the 2 tags.
Returns : Nothing.
Note : Method(s) that call(s) this method : Many.
Method(s) that this method call(s) : None.
=cut
sub
_helper_store_attribute_list {
my
(
$self
,
$attribute_line
,
$data_structure
) =
@_
;
my
%attribs
= (
$attribute_line
=~ /(\w+)\s*=\s*
"([^"
]*)"/g);
my
$attribute_list
;
for
my
$key
(
keys
%attribs
) {
# print "\tkey: $key , value: $attribs{$key}\n";
###$$data_structure->{$key} = $attribs{$key}; # <- The ORIGINAL.
push
@{
$$data_structure
->{
$key
}},
$attribs
{
$key
};
# Now, store them in an array because there may be > 1 tag, thus
# > 1 attribute of the same name.
# Doing this has made it necessary to change the _store_seqs method.
# ie: Change $bio_sequence->{'molecule_type'};
# to
# $bio_sequence->{'molecule_type'}->[0];
}
return
;
}
# ==================================================================================
=head2 _store_seqs
Title : _store_seqs
Usage : $self->_store_seqs
Function : This method is called once in the life time of the script.
It stores the data parsed from the agave xml file into
the FAST::Bio::Seq object.
Args : None.
Returns : Nothing.
Note : Method(s) that call(s) this method : next_seq
Method(s) that this method calls : None.
=cut
sub
_store_seqs {
my
(
$self
) =
@_
;
for
my
$sciobj
(@{
$self
->{
'sciobj'
}}) {
### $sciobj = $self->{'sciobj'}; # The root node.
for
my
$contig
(@{
$sciobj
->{
'contig'
}}) {
# Each contig has a fragment order.
for
my
$fragment_order
(@{
$contig
->{
'fragment_order'
}}) {
# Each fragment order has a fragment_orientation.
for
my
$fragment_orientation
(@{
$fragment_order
->{
'fragment_orientation'
}}) {
# Each fragment_orientation contain 1 bio sequence.
my
$bio_sequence
=
$fragment_orientation
->{
'bio_sequence'
};
# <bio_sequence> contains all the
# interesting stuff:
my
$sequence
=
$bio_sequence
->{
'sequence'
};
my
$accession_number
=
$bio_sequence
->{
'sequence_id'
}->[0];
# also use for primary_id
my
$organism
=
$bio_sequence
->{
'organism'
};
my
$description
=
$bio_sequence
->{
'description'
};
my
$molecule_type
=
$bio_sequence
->{
'molecule_type'
}->[0];
my
$primary_seq
= FAST::Bio::PrimarySeq->new(
-id
=>
$accession_number
,
-alphabet
=>
$molecule_type
,
-seq
=>
$sequence
,
-desc
=>
$description
,
);
my
$seq
= FAST::Bio::Seq->new (
-display_id
=>
$accession_number
,
-accession_number
=>
$accession_number
,
-primary_seq
=>
$primary_seq
,
-seq
=>
$sequence
,
-description
=>
$description
,
);
my
$organism_name
=
$bio_sequence
->{organism_name}->[0];
if
(
defined
$organism_name
) {
my
@classification
=
split
(
' '
,
$organism_name
);
my
$species
= FAST::Bio::Species->new();
$species
->classification(
@classification
);
$seq
->species(
$species
);
}
# Pull out the keywords: $keywords is an array ref.
my
$keywords
=
$bio_sequence
->{keyword};
my
%key_to_value
;
for
my
$keywords
(
@$keywords
) {
# print "keywords: $keywords\n";
my
@words
=
split
(
':'
,
$keywords
);
for
(
my
$i
= 0;
$i
<
scalar
@words
- 1;
$i
++) {
if
(
$i
% 2 == 0) {
my
$j
=
$i
;
$j
++;
# print "$words[$i] , $words[$j]\n";
$key_to_value
{
$words
[
$i
]} =
$words
[
$j
];
}
}
# print Data::Dumper->Dump([%key_to_value]);
my
$reference
= FAST::Bio::Annotation::Reference->
new(
-authors
=>
$key_to_value
{authors},
-title
=>
$key_to_value
{title},
-database
=>
$key_to_value
{database},
-pubmed
=>
$key_to_value
{pubmed},
);
$seq
->annotation->add_Annotation(
'reference'
,
$reference
);
}
# close for my $keywords
# print Data::Dumper->Dump([$bio_sequence]); print "here\n"; exit;
if
(
defined
$bio_sequence
->{
'sequence_map'
}) {
for
my
$sequence_map
(@{
$bio_sequence
->{
'sequence_map'
}}) {
# print Data::Dumper->Dump([$sequence_map]); print "here\n"; exit;
my
$label
=
$sequence_map
->{label};
if
(
defined
$sequence_map
->{annotations} &&
ref
(
$sequence_map
->{annotations}) eq
'HASH'
) {
# Get the sequence features (ie genes, exons, etc) from this $sequence_map
for
my
$seq_feature
(@{
$sequence_map
->{
'annotations'
}->{
'seq_feature'
}}) {
# print Data::Dumper->Dump([$seq_feature]); exit;
my
$seq_location
=
$seq_feature
->{
'seq_location'
};
my
$start_coord
=
$seq_feature
->{
'least_start'
}->[0];
my
$feature_type
=
$seq_feature
->{
'feature_type'
}->[0];
my
$end_coord
=
$seq_feature
->{
'greatest_end'
}->[0];
my
$is_on_complement
=
$seq_feature
->{
'is_on_complement'
}->[0];
# Specify the coordinates and the tag for this seq feature.
# print "Primary Tag for this SeqFeature: $feature_type\n";
my
$feat
= FAST::Bio::SeqFeature::Generic->
new(
-start
=>
$start_coord
,
-end
=>
$end_coord
,
-primary_tag
=>
$feature_type
,
);
if
(
defined
$seq_feature
->{
'qualifier'
} &&
ref
(
$seq_feature
->{
'qualifier'
}) eq
'ARRAY'
) {
for
my
$feature
(@{
$seq_feature
->{
'qualifier'
}}) {
my
$value
=
$feature
->{
'qualifier'
};
my
$feature_type
=
$feature
->{
'qualifier_type'
};
for
(
my
$i
= 0;
$i
<
scalar
@{
$value
};
$i
++) {
$feat
->add_tag_value(
$feature_type
->[
$i
] =>
$value
->[
$i
]
);
}
# close the for loop
}
}
# close if (defined $seq_feature->...
$seq
->add_SeqFeature(
$feat
);
}
# close for my $seq_feature (@{$sequence_map->...
}
# close if (defined $sequence_map->{annotations} &&
}
# close for my $sequence_map (@{$bio_sequence->{'sequence_map'}}){
}
# close if (defined $bio_sequence->{'sequence_map'}){
# This is where the FAST::Bio::Seq objects are stored:
push
@{
$self
->{
'sequence_objects'
}},
$seq
;
}
# close for my $fragment_orientation
}
# close for my $fragment_order
}
# close for my $contig
}
# close for my $sciobj
# Flag is set so that we know that the sequence objects are now stored in $self.
$self
->{
'seqs_stored'
} = 1;
return
;
}
# ==================================================================================
=head2 next_seq
Title : next_seq
Usage : $seq = $stream->next_seq()
Function : Returns the next sequence in the stream.
Args : None.
Returns : FAST::Bio::Seq object
Method is called from the script. Method(s) that this method calls:
_store_seqs (only once throughout the life time of script execution).
=cut
sub
next_seq {
my
(
$self
) =
@_
;
# convert agave to genbank/fasta/embl whatever.
$self
->_store_seqs
if
$self
->{
'seqs_stored'
} == 0;
$self
->throw(
"Error: No FAST::Bio::Seq objects stored yet!\n\n"
)
if
!
defined
$self
->{
'sequence_objects'
};
# This should never occur...
if
(
scalar
@{
$self
->{
'sequence_objects'
}} > 0) {
return
shift
@{
$self
->{
'sequence_objects'
}};
}
else
{
# All done. Nothing more to parse.
# print "returning nothing!\n";
return
0;
}
}
# ==================================================================================
=head2 next_primary_seq
Title : next_primary_seq
Usage : $seq = $stream->next_primary_seq()
Function: returns the next primary sequence (ie no seq_features) in the stream
Returns : FAST::Bio::PrimarySeq object
Args : NONE
=cut
sub
next_primary_seq {
my
$self
=
shift
;
return
0;
}
# ==================================================================================
=head2 write_seq
Title : write_seq
Usage : Not Yet Implemented! $stream->write_seq(@seq)
Function: writes the $seq object into the stream
Returns : 1 for success and 0 for error
Args : FAST::Bio::Seq object
=cut
sub
write_seq {
# Convert the FAST::Bio::Seq object(s) to AGAVE xml file.
my
(
$self
,
@seqs
) =
@_
;
foreach
my
$seq
(
@seqs
) {
$self
->_write_each_record(
$seq
);
# where most of the work actually takes place.
}
return
;
}
# ==================================================================================
=head2 _write_each_record
Title : _write_each_record
Usage : $agave->_write_each_record( $seqI )
Function: change data into agave format
Returns : NONE
Args : FAST::Bio::SeqI object
=cut
sub
_write_each_record {
my
(
$self
,
$seq
) =
@_
;
# $self->{'file'} =~ s/>//g;
my
$output
= IO::File->new(
">"
.
$self
->{
'file'
});
my
$writer
= XML::Writer->new(
OUTPUT
=>
$output
,
NAMESPACES
=> 0,
DATA_MODE
=> 1,
DATA_INDENT
=> 2 ) ;
$writer
->xmlDecl(
"UTF-8"
);
$writer
->doctype(
"sciobj"
,
''
,
"sciobj.dtd"
);
$writer
->startTag(
'sciobj'
,
'version'
,
'2'
,
'release'
,
'2'
);
$writer
->startTag(
'contig'
,
'length'
,
$seq
->
length
);
my
$annotation
=
$seq
->annotation;
# print "annotation: $annotation\n"; exit; FAST::Bio::Annotation::Collection=HASH(0x8112e6c)
if
(
$annotation
->get_Annotations(
'dblink'
) ) {
# used to be $annotation->each_DBLink, but FAST::Bio::Annotation::Collection::each_DBLink
# is now replaced with get_Annotations('dblink')
my
$dblink
=
$annotation
->get_Annotations(
'dblink'
)->[0] ;
$writer
->startTag(
'db_id'
,
'id'
,
$dblink
->primary_id ,
'db_code'
,
$dblink
->database );
}
else
{
$writer
->startTag(
'db_id'
,
'id'
,
$seq
->display_id ,
'db_code'
,
'default'
);
}
$writer
->endTag(
'db_id'
) ;
$writer
->startTag(
'fragment_order'
);
$writer
->startTag(
'fragment_orientation'
);
##start bio_sequence
####my $organism = $seq->species->genus . " " . $seq->species->species;
$writer
->startTag(
'bio_sequence'
,
'sequence_id'
,
$seq
->display_id,
'seq_length'
,
$seq
->
length
,
# 'molecule_type', $seq->moltype, # deprecated
'molecule_type'
,
$self
->alphabet,
#'organism_name', $organism
);
# my $desc = $seq->{primary_seq}->{desc};
# print "desc: $desc\n"; exit;
# print Data::Dumper->Dump([$seq]); exit;
##start db_id under bio_sequence
$annotation
=
$seq
->annotation;
# print "annotation: $annotation\n"; exit; FAST::Bio::Annotation::Collection=HASH(0x8112e6c)
if
(
$annotation
->get_Annotations(
'dblink'
) ) {
# used to be $annotation->each_DBLink, but FAST::Bio::Annotation::Collection::each_DBLink
# is now replaced with get_Annotations('dblink')
my
$dblink
=
$annotation
->get_Annotations(
'dblink'
)->[0] ;
$writer
->startTag(
'db_id'
,
'id'
,
$dblink
->primary_id ,
'db_code'
,
$dblink
->database );
}
else
{
$writer
->startTag(
'db_id'
,
'id'
,
$seq
->display_id ,
'db_code'
,
'default'
);
}
$writer
->endTag(
'db_id'
) ;
##start note
my
$note
=
""
;
foreach
my
$comment
(
$annotation
->get_Annotations(
'comment'
) ) {
# used to be $annotations->each_Comment(), but that's now been replaced
# with get_Annotations()
# $comment is a FAST::Bio::Annotation::Comment object
$note
.=
$comment
->text() .
"\n"
;
}
$writer
->startTag(
'note'
);
$writer
->characters(
$note
) ;
$writer
->endTag(
'note'
);
##start description
$writer
->startTag(
'description'
);
# $writer ->characters( $annotation->get_Annotations('description') ) ;
# used to be $annotations->each_description(), but that's now been
# replaced with get_Annotations.
# Simon added this: this is the primary_seq's desc (the DEFINITION tag in a genbank file)
$writer
->characters(
$seq
->{primary_seq}->{desc});
$writer
->endTag(
'description'
);
##start keywords
foreach
my
$genename
(
$annotation
->get_Annotations(
'gene_name'
) ) {
# used to be $annotations->each_gene_name, but that's now been
# replaced with get_Annotations()
$writer
->startTag(
'keyword'
);
$writer
->characters(
$genename
) ;
$writer
->endTag(
'keyword'
);
}
foreach
my
$ref
(
$annotation
->get_Annotations(
'reference'
) ) {
# used to be $annotation->each_Reference, but
# that's now been replaced with get_Annotations('reference');
# link is a FAST::Bio::Annotation::Reference object
$writer
->startTag(
'keyword'
);
# print Data::Dumper->Dump([$ref]); exit;
my
$medline
=
$ref
->medline ||
'null'
;
my
$pubmed
=
$ref
->pubmed ||
'null'
;
my
$database
=
$ref
->database ||
'null'
;
my
$authors
=
$ref
->authors ||
'null'
;
my
$title
=
$ref
->title ||
'null'
;
$writer
->characters(
'medline:'
.
"$medline"
.
':'
.
'pubmed:'
.
"$pubmed"
.
':'
.
'database:'
.
"$database"
.
':'
.
'authors:'
.
"$authors"
.
':'
.
'title:'
.
"$title"
) ;
$writer
->endTag(
'keyword'
);
}
## start sequence
$writer
->startTag(
'sequence'
);
$writer
->characters(
$seq
->seq ) ;
$writer
->endTag(
'sequence'
);
## start xrefs
$writer
->startTag(
'xrefs'
);
foreach
my
$link
(
$annotation
->get_Annotations(
'dblink'
) ) {
# link is a FAST::Bio::Annotation::DBLink object
$writer
->startTag(
'db_id'
,
'db_code'
,
$link
->database,
'id'
,
$link
->primary_id);
$writer
->characters(
$link
->comment ) ;
$writer
->endTag(
'db_id'
);
}
$writer
->endTag(
'xrefs'
) ;
##start sequence map
##we can not use : my @feats = $seq->all_SeqFeatures;
##rather, we use top_SeqFeatures() to keep the tree structure
my
@feats
=
$seq
->top_SeqFeatures ;
my
$features
;
##now we need cluster top level seqfeature by algorithm
my
$maps
;
foreach
my
$feature
(
@feats
) {
my
$map_type
=
$feature
->source_tag;
push
(@{
$maps
->{
$map_type
}},
$feature
);
}
##now we enter each sequence_map
foreach
my
$map_type
(
keys
%$maps
) {
$writer
->startTag(
'sequence_map'
,
'label'
,
$map_type
);
$writer
->startTag(
'annotations'
);
# the original author accidently entered 'annotation' instead of 'annotations'
foreach
my
$feature
( @{
$maps
->{
$map_type
}} ) {
$self
->_write_seqfeature(
$feature
,
$writer
) ;
}
$writer
->endTag(
'annotations'
);
$writer
->endTag(
'sequence_map'
);
}
$writer
->endTag(
'bio_sequence'
);
$writer
->endTag(
'fragment_orientation'
);
$writer
->endTag(
'fragment_order'
);
$writer
->endTag(
'contig'
);
$writer
->endTag(
'sciobj'
);
}
# ==================================================================================
=head2 _write_seqfeature
Usage : $agave->_write_each_record( $seqfeature, $write )
Function: change seeqfeature data into agave format
Returns : NONE
Args : FAST::Bio::SeqFeature object and XML::writer object
=cut
sub
_write_seqfeature{
my
(
$self
,
$seqf
,
$writer
) =
@_
;
##now enter seq feature
$writer
->startTag(
'seq_feature'
,
'feature_type'
,
$seqf
->primary_tag() );
my
$strand
=
$seqf
->strand();
$strand
= 0
if
!
defined
$strand
;
# $strand == 1 ? 'false' : 'true';
my
$is_on_complement
;
if
(
$strand
== 1) {
$is_on_complement
=
'true'
;
}
else
{
$is_on_complement
=
'false'
;
}
# die Data::Dumper->Dump([$seqf]) if !defined $strand;
$writer
->startTag(
'seq_location'
,
'lease_start'
,
$seqf
->start(),
'greatest_end'
,
$seqf
->end(),
# 'is_on_complement', $seqf->strand() == 1 ? 'false' : 'true') ;
'is_on_complement'
,
$is_on_complement
);
# is_on_complement: is the feature found on the complementary
# strand (true) or not (false)?
$writer
->endTag(
'seq_location'
);
##enter qualifier
foreach
my
$tag
(
$seqf
->all_tags() ) {
$writer
->startTag(
'qualifier'
,
'qualifier_type'
,
$tag
);
$writer
->characters(
$seqf
->each_tag_value(
$tag
) ) ;
$writer
->endTag(
'qualifier'
);
}
##now recursively travel the seqFeature
foreach
my
$subfeat
(
$seqf
->sub_SeqFeature ) {
$self
->_write_seqfeature(
$subfeat
,
$writer
) ;
}
$writer
->endTag(
'seq_feature'
);
return
;
}
# ==================================================================================
=head2 _filehandle
Title : _filehandle
Usage : $obj->_filehandle($newval)
Function:
Example :
Returns : value of _filehandle
Args : newvalue (optional)
=cut
sub
_filehandle{
my
(
$obj
,
$value
) =
@_
;
if
(
defined
$value
) {
$obj
->{
'_filehandle'
} =
$value
;
}
return
$obj
->{
'_filehandle'
};
}
# ==================================================================================
=head2 throw
Title : throw
Usage : $self->throw;
Function : Throw's error message. Calls SeqIO's throw method.
Args : Array of string(s), holding error message(s).
Returns : Nothing.
Note : Method(s) that call(s) this method: many.
Method(s) that this method calls: FAST::Bio::SeqIO's throw method.
=cut
sub
throw {
my
(
$self
,
@s
) =
@_
;
my
$string
=
"[$.]"
.
join
(
''
,
@s
);
$self
->SUPER::throw(
$string
);
return
;
}
1;