#
# BioPerl module for FAST::Bio::SearchIO::hmmer
#
# Please direct questions and support issues to <bioperl-l@bioperl.org>
#
# Cared for by Kai Blin <kai.blin@biotech.uni-tuebingen.de>
#
# Copyright Kai Blin
#
# You may distribute this module under the same terms as perl itself
#
# POD documentation - main docs before the code
=head1 NAME
FAST::Bio::SearchIO::hmmer - A parser for HMMER2 and HMMER3 output (hmmscan, hmmsearch, hmmpfam)
=head1 SYNOPSIS
# do not use this class directly it is available through FAST::Bio::SearchIO
use FAST::Bio::SearchIO;
my $in = FAST::Bio::SearchIO->new(-format => 'hmmer',
-file => 't/data/L77119.hmmer');
while( my $result = $in->next_result ) {
# this is a FAST::Bio::Search::Result::HMMERResult object
print $result->query_name(), " for HMM ", $result->hmm_name(), "\n";
while( my $hit = $result->next_hit ) {
print $hit->name(), "\n";
while( my $hsp = $hit->next_hsp ) {
print "length is ", $hsp->length(), "\n";
}
}
}
=head1 DESCRIPTION
This object implements a parser for HMMER output. It works with both HMMER2 and HMMER3
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via the
web:
=head1 AUTHOR - Kai Blin
Email kai.blin-at-biotech.uni-tuebingen.de
=head1 APPENDIX
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
=cut
# Let the code begin...
use strict;
sub new {
my ( $caller, @args ) = @_;
my $class = ref($caller) || $caller;
if( $class =~ /FAST::Bio::SearchIO::hmmer(\d)/ ) {
my ($self) = $class->SUPER::new(@args);
$self->_initialize(@args);
return $self;
} else {
# Try to guess the hmmer format version if it's not specified.
my $version;
my $fh;
my $fh_needs_close = 0;
my %param = @args;
@param{ map { lc $_ } keys %param } = values %param; # lowercase keys
# If the caller specified a version, go for that
if (defined($param{"-version"})) {
$version = $param{"-version"};
} else {
# Check the contents of the file for the hmmer version mentioned in there
if (defined($param{"-fh"})) {
$fh = $param{"-fh"};
} elsif (defined($param{"-file"})) {
open($fh, $param{"-file"}) || return;
$fh_needs_close = 1;
}
# read second line of the file
<$fh>;
$_ = <$fh>;
if ( m/HMMER\s3/ ) {
$version = "3";
} else {
$version = "2";
}
if ($fh_needs_close) {
close($fh);
} else {
seek($fh, 0, 0);
}
}
my $format = "hmmer$version";
return unless( $class->_load_format_module($format) );
return "FAST::Bio::SearchIO::${format}"->new(@args);
}
}
sub _initialize {
my ( $self, @args ) = @_;
$self->SUPER::_initialize(@args);
my $handler = $self->_eventHandler;
$handler->register_factory(
'result',
FAST::Bio::Factory::ObjectFactory->new(
-type => 'FAST::Bio::Search::Result::HMMERResult',
-interface => 'FAST::Bio::Search::Result::ResultI'
)
);
$handler->register_factory(
'hit',
FAST::Bio::Factory::ObjectFactory->new(
-type => 'FAST::Bio::Search::Hit::HMMERHit',
-interface => 'FAST::Bio::Search::Hit::HitI'
)
);
$handler->register_factory(
'hsp',
FAST::Bio::Factory::ObjectFactory->new(
-type => 'FAST::Bio::Search::HSP::HMMERHSP',
-interface => 'FAST::Bio::Search::HSP::HSPI'
)
);
}
1;