—
—
—
—

              
# $Id: chaos.pm,v 1.10.4.1 2006/10/02 23:10:28 sendu Exp $
# $Date: 2006/10/02 23:10:28 $
#
# BioPerl module for Bio::SeqIO::chaos
#
# Chris Mungall <cjm@fruitfly.org>
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
HideShow 105 lines of Pod
=head1 NAME
Bio::SeqIO::chaos - chaos sequence input/output stream
=head1 SYNOPSIS
    #In general you will not want to use this module directly;
    #use the chaosxml format via SeqIO
    $outstream = Bio::SeqIO->new(-file => $filename,
                                 -format => 'chaosxml');
    while ( my $seq = $instream->next_seq() ) {
       $outstream->write_seq($seq);
    }
=head1 DESCRIPTION
This is the guts of L<Bio::SeqIO::chaosxml> - please refer to the
documentation for this module
B<CURRENTLY WRITE ONLY>
ChaosXML is an XML mapping of the chado relational database; for more
information, see http://www.fruitfly.org/chaos-xml
chaos can be represented in various syntaxes - XML, S-Expressions or
indented text. You should see the relevant SeqIO file. You will
probably want to use L<Bio::SeqIO::chaosxml>, which is a wrapper to
this module.
=head2 USING STAG OBJECTS
B<non-standard bioperl stuff you dont necessarily need to know follows>
This module (in write mode) is an B<event producer> - it generates XML
events via the L<Data::Stag> module. If you only care about the final
end-product xml, use L<Bio::SeqIO::chaosxml>
You can treat the resulting chaos-xml stream as stag XML objects;
    $outstream = Bio::SeqIO->new(-file => $filename, -format => 'chaos');
    while ( my $seq = $instream->next_seq() ) {
       $outstream->write_seq($seq);
    }
    my $chaos = $outstream->handler->stag;
    # stag provides get/set methods for xml elements
    # (these are chaos objects, not bioperl objects)
    my @features = $chaos->get_feature;
    my @feature_relationships = $chaos->get_feature_relationships;
    # stag objects can be queried with functional-programming
    # style queries
    my @features_in_range =
      $chaos->where('feature',
                    sub {
                         my $featureloc = shift->get_featureloc;
                         $featureloc->strand == 1 &&
                         $featureloc->nbeg > 10000 &&
                         $featureloc->nend < 20000;
                    });
    foreach my $feature (@features_in_range) {
      my $featureloc = $feature->get_featureloc;
      printf "%s [%d->%d on %s]\n",
        $feature->sget_name,
        $featureloc->sget_nbeg,
        $featureloc->sget_end,
        $featureloc->sget_srcfeature_id;
    }
=head1 MODULES REQUIRED
L<Data::Stag>
Downloadable from CPAN; see also http://stag.sourceforge.net
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists.  Your participation is much appreciated.
  bioperl-l@bioperl.org                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution.
Bug reports can be submitted via the web:
  http://bugzilla.bioperl.org
=head1 AUTHOR - Chris Mungall
Email cjm@fruitfly.org
=head1 APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
=cut
# Let the code begin...
package Bio::SeqIO::chaos;
use strict;
use Bio::SeqFeature::Generic;
use Bio::Species;
use Bio::Seq::SeqFactory;
use Bio::Annotation::Collection;
use Bio::Annotation::Comment;
use Bio::Annotation::Reference;
use Bio::Annotation::DBLink;
use Bio::SeqFeature::Tools::TypeMapper;
use Bio::SeqFeature::Tools::FeatureNamer;
use Bio::SeqFeature::Tools::IDHandler;
use Data::Stag qw(:all);
use base qw(Bio::SeqIO);
our $TM = 'Bio::SeqFeature::Tools::TypeMapper';
our $FNAMER = 'Bio::SeqFeature::Tools::FeatureNamer';
our $IDH = 'Bio::SeqFeature::Tools::IDHandler';
sub _initialize {
    my($self,@args) = @_;
    $self->SUPER::_initialize(@args);
    if( ! defined $self->sequence_factory ) {
        $self->sequence_factory(new Bio::Seq::SeqFactory
                                (-verbose => $self->verbose(),
                                 -type => 'Bio::Seq::RichSeq'));
    }
    my $wclass = $self->default_handler_class;
    $self->handler($wclass);
    if ($self->_fh) {
        $self->handler->fh($self->_fh);
    }
    $self->{_end_of_data} = 0;
    $self->_type_by_id_h({});
    my $t = time;
    my $ppt = localtime $t;
    $self->handler->S("chaos");
    $self->handler->ev(chaos_metadata=>[
                                        [chaos_version=>1],
                                        [chaos_flavour=>'bioperl'],
                                        [feature_unique_key=>'feature_id'],
                                        [equiv_chado_release=>'chado_1_01'],
                                        [export_unixtime=>$t],
                                        [export_localtime=>$ppt],
                                        [export_host=>$ENV{HOST}],
                                        [export_user=>$ENV{USER}],
                                        [export_perl5lib=>$ENV{PERL5LIB}],
                                        [export_program=>$0],
                                        [export_module=>'Bio::SeqIO::chaos'],
                                        [export_module_cvs_id=>'$Id: chaos.pm,v 1.10.4.1 2006/10/02 23:10:28 sendu Exp $'],
                                       ]);
    return;
}
sub DESTROY {
    my $self = shift;
    $self->end_of_data();
    $self->SUPER::DESTROY();
}
sub end_of_data {
    my $self = shift;
    return if $self->{_end_of_data};
    $self->{_end_of_data} = 1;
    $self->handler->E("chaos");
}
sub default_handler_class {
    return Data::Stag->makehandler;
}
HideShow 13 lines of Pod
=head2 context_namespace
 Title   : context_namespace
 Usage   : $obj->context_namespace($newval)
 Function:
 Example :
 Returns : value of context_namespace (a scalar)
 Args    : on set, new value (a scalar or undef, optional)
IDs will be preceeded with the context namespace
=cut
sub context_namespace{
    my $self = shift;
    return $self->{'context_namespace'} = shift if @_;
    return $self->{'context_namespace'};
}
HideShow 10 lines of Pod
=head2 next_seq
 Title   : next_seq
 Usage   : $seq = $stream->next_seq()
 Function: returns the next sequence in the stream
 Returns : Bio::Seq object
 Args    :
=cut
sub next_seq {
    my ($self,@args) = @_;
    my $seq = $self->sequence_factory->create
        (
         #         '-verbose' =>$self->verbose(),
         #       %params,
         #       -seq => $seqc,
         #       -annotation => $annotation,
         #       -features => \@features
        );
    return $seq;
}
sub handler {
    my $self = shift;
    $self->{_handler} = shift if @_;
    return $self->{_handler};
}
HideShow 11 lines of Pod
=head2 write_seq
 Title   : write_seq
 Usage   : $stream->write_seq($seq)
 Function: writes the $seq object (must be seq) to the stream
 Returns : 1 for success and 0 for error
 Args    : Bio::Seq
=cut
sub write_seq {
    my ($self,$seq) = @_;
    if( !defined $seq ) {
        $self->throw("Attempting to write with no seq!");
    }
    if( ! ref $seq || ! $seq->isa('Bio::SeqI') ) {
        $self->warn(" $seq is not a SeqI compliant module. Attempting to dump, but may fail!");
    }
    # get a handler - must inherit from Data::Stag::BaseHandler;
    my $w = $self->handler;
    # start of data
    ###    $w->S("chaos_block");
    my $seq_chaos_feature_id;
    # different seq objects have different version accessors -
    # weird but true
    my $version = $seq->can('seq_version') ? $seq->seq_version : $seq->version;
    my $accversion = $seq->accession_number;
    if ($version) {
        $accversion .= ".$version";
    }
    if ($accversion) {
        $seq_chaos_feature_id = $accversion;
    }
    else {
        $seq_chaos_feature_id = $self->get_chaos_feature_id($seq);
        $accversion = $seq_chaos_feature_id;
    }
    # All ids must have a namespace prefix
    if ($seq_chaos_feature_id !~ /:/) {
        $seq_chaos_feature_id = "GenericSeqDB:$seq_chaos_feature_id";
    }
#    if ($seq->accession_number eq 'unknown') {
#        $seq_chaos_feature_id = $self->get_chaos_feature_id('contig', $seq);
#    }
    my $haplotype;
    if ($seq->desc =~ /haplotype(.*)/i) {
        # yikes, no consistent way to specify haplotype in gb
        $haplotype = $1;
        $haplotype =~ s/\s+/_/g;
        $haplotype =~ s/\W+//g;
    }
    my $OS;
    # Organism lines
    if (my $spec = $seq->species) {
        my ($species, $genus, @class) = $spec->classification();
        $OS = "$genus $species";
        if (my $ssp = $spec->sub_species) {
            $OS .= " $ssp";
        }
        $self->genus_species($OS);
        if( $spec->common_name ) {
            my $common = $spec->common_name;
            # genbank parser sets species->common_name to
            # be "Genus Species (common name)" which is wrong;
            # we will correct for this; if common_name is set
            # correctly then carry on
            if ($common =~ /\((.*)\)/) {
                $common = $1;
            }
            $OS .= " (".$common.")";
        }
    }
    if ($OS) {
        $self->organismstr($OS);
    }
    if ($haplotype) {
        # genus_species is part of uniquename - add haplotype
        # to make it genuinely unique
        $self->genus_species($self->genus_species .= " $haplotype");
    }
    my $uname = $self->make_uniquename($self->genus_species, $accversion);
    # data structure representing the core sequence for this record
    my $seqnode =
      Data::Stag->new(feature=>[
                                [feature_id=>$seq_chaos_feature_id],
                                [dbxrefstr=>'SEQDB:'.$accversion],
                                [name=>$seq->display_name],
                                [uniquename=>$uname],
                                [residues=>$seq->seq],
                               ]);
    # soft properties
    my %prop = ();
    $seqnode->set_type('databank_entry');
    map {
        $prop{$_} = $seq->$_() if $seq->can($_);
    } qw(desc keywords division molecule is_circular);
    $prop{dates} = join("; ", $seq->get_dates) if $seq->can("get_dates");
    local($^W) = 0;   # supressing warnings about uninitialized fields.
    # Reference lines
    my $count = 1;
    foreach my $ref ( $seq->annotation->get_Annotations('reference') ) {
        # TODO
    }
    # Comment lines
    $seqnode->add_featureprop([[type=>'haplotype'],[value=>$haplotype]])
      if $haplotype;
    foreach my $comment ( $seq->annotation->get_Annotations('comment') ) {
        $seqnode->add_featureprop([[type=>'comment'],[value=>$comment->text]]);
    }
    if ($OS) {
        $seqnode->set_organismstr($OS);
    }
    my @sfs = $seq->get_SeqFeatures;
    # genbank usually includes a 'source' feature - we just
    # migrate the data from this to the actual source feature
    my @sources = grep {$_->primary_tag eq 'source'} @sfs;
    @sfs = grep {$_->primary_tag ne 'source'} @sfs;
    $self->throw(">1 source types") if @sources > 1;
    my $source = shift @sources;
    if ($source) {
        my $tempw = Data::Stag->makehandler;
        $self->write_sf($source, $seq_chaos_feature_id, $tempw);
        my $snode = $tempw->stag;
        $seqnode->add($_->name, $_->data)
          foreach ($snode->get_featureprop,
                   $snode->get_feature_dbxref);
    }
    # throw the writer an event
    $w->ev(@$seqnode);
    $seqnode = undef;      # free memory
    # make events for all the features within the record
    foreach my $sf ( @sfs ) {
        $FNAMER->name_feature($sf);
        $FNAMER->name_contained_features($sf);
        $self->write_sf($sf, $seq_chaos_feature_id);
    }
    # data end
    ### $w->E("chaos_block");
    return 1;
}
sub organismstr{
    my $self = shift;
    return $self->{'organismstr'} = shift if @_;
    return $self->{'organismstr'};
}
sub genus_species{
    my $self = shift;
    return $self->{'genus_species'} = shift if @_;
    return $self->{'genus_species'};
}
# maps ID to type
sub _type_by_id_h {
    my $self = shift;
    $self->{_type_by_id_h} = shift if @_;
    return $self->{_type_by_id_h};
}
# ----
# writes a seq feature
# ----
sub write_sf {
    my $self = shift;
    my $sf = shift;
    my $seq_chaos_feature_id = shift;
    my $w = shift || $self->handler;
    my %props =
      map {
          lc($_)=>[$sf->each_tag_value($_)]
      } $sf->all_tags;
    my $loc = $sf->location;
    my $name = $FNAMER->generate_feature_name($sf);
    my $type = $sf->primary_tag;
    # The CDS (eg in a genbank feature) implicitly represents
    # the protein
    $type =~ s/CDS/polypeptide/;
    my @subsfs = $sf->sub_SeqFeature;
    my @locnodes = ();
    my $sid = $loc->is_remote ? $loc->seq_id : $seq_chaos_feature_id;
    my $CREATE_SPLIT_SFS = 0;
    if($CREATE_SPLIT_SFS &&
       $loc->isa("Bio::Location::SplitLocationI") ) {
        # turn splitlocs into subfeatures
        my $n = 1;
        push(@subsfs,
             map {
                 my $ssf =
                   Bio::SeqFeature::Generic->new(
                                                 -start=>$_->start,
                                                 -end=>$_->end,
                                                 -strand=>$_->strand,
                                                 -primary=>$self->subpartof($type),
                                              );
                 if ($_->is_remote) {
                     $ssf->location->is_remote(1);
                     $ssf->location->seq_id($_->seq_id);
                 }
                 $ssf;
               } $loc->each_Location);
    }
    elsif( $loc->isa("Bio::Location::RemoteLocationI") ) {
        # turn splitlocs into subfeatures
        my $n = 1;
        push(@subsfs,
             map {
                 Bio::SeqFeature::Generic->new(
#                                               -name=>$name.'.'.$n++,
                                               -start=>$_->start,
                                               -end=>$_->end,
                                               -strand=>$_->strand,
                                               -primary=>$self->subpartof($type),
                                              )
               } $loc->each_Location);
    }
    else {
        my ($beg, $end, $strand) = $self->bp2ib($loc);
        if (!$strand) {
            use Data::Dumper;
            print Dumper $sf, $loc;
            $self->throw("($beg, $end, $strand) - no strand\n");
        }
        @locnodes = (
                     [featureloc=>[
                                   [nbeg=>$beg],
                                   [nend=>$end],
                                   [strand=>$strand],
                                   [srcfeature_id=>$sid],
                                   [locgroup=>0],
                                   [rank=>0],
                                  ]
                     ]
                    );
    }
    my $feature_id = $self->get_chaos_feature_id($sf);
    delete $props{id} if $props{id};
    # do something with genbank stuff
    my $pid = $props{'protein_id'};
    my $tn = $props{'translation'};
    my @xrefs = @{$props{'db_xref'} || []};
    if ($pid) {
        push(@xrefs, "protein:$pid->[0]");
    }
    my $org = $props{organism} ? $props{organism}->[0] : undef;
    if (!$org && $self->organismstr) {
        $org = $self->organismstr;
    }
    my $uname = $name ? $name.'/'.$feature_id : $feature_id;
    if ($self->genus_species && $name) {
        $uname = $self->make_uniquename($self->genus_species, $name);
    }
    if (!$uname) {
        $self->throw("cannot make uniquename for $feature_id $name");
    }
    $self->_type_by_id_h->{$feature_id} = $type;
    my $fnode =
      [feature=>[
                 [feature_id=>$feature_id],
                 $name ? ([name=>$name]) : (),
                 [uniquename=>$uname],
                 [type=>$type],
                 $tn ? ([residues=>$tn->[0]],
                        [seqlen=>length($tn->[0])],
                        #####[md5checksum=>md5checksum($tn->[0])],
                       ) :(),
                 $org ? ([organismstr=>$org]) : (),
                 @locnodes,
                 (map {
                     [feature_dbxref=>[
                                       [dbxrefstr=>$_]
                                      ]
                     ]
                 } @xrefs),
                 (map {
                     my $k = $_;
                     my $rank=0;
                     map { [featureprop=>[[type=>$k],[value=>$_],[rank=>$rank++]]] } @{$props{$k}}
                 } keys %props),
                ]];
    $w->ev(@$fnode);
    my $rank = 0;
    if (@subsfs) {
        # strand is always determined by FIRST feature listed
        # (see genbank entry for trans-spliced mod(mdg4) AE003734)
        my $strand = $subsfs[0];
        # almost all the time, all features are on same strand
        my @sfs_on_main_strand = grep {$_->strand == $strand} @subsfs;
        my @sfs_on_other_strand = grep {$_->strand != $strand} @subsfs;
        sort_by_strand($strand, \@sfs_on_main_strand);
        sort_by_strand(0-$strand, \@sfs_on_other_strand);
        @subsfs = (@sfs_on_main_strand, @sfs_on_other_strand);
        foreach my $ssf (@subsfs) {
            my $ssfid = $self->write_sf($ssf, $sid);
            #my $rtype = 'part_of';
            my $rtype =
              $TM->get_relationship_type_by_parent_child($sf,$ssf);
            if ($ssf->primary_tag eq 'CDS') {
                $rtype = 'derives_from';
            }
            $w->ev(feature_relationship=>[
                                          [subject_id=>$ssfid],
                                          [object_id=>$feature_id],
                                          [type=>$rtype],
                                          [rank=>$rank++],
                                         ]
                  );
        }
    }
    else {
        # parents not stored as bioperl containment hierarchy
        my @parent_ids = @{$props{parent} || []};
        foreach my $parent_id (@parent_ids) {
            my $ptype =
              $self->_type_by_id_h->{$parent_id} || 'unknown';
            my $rtype =
              $TM->get_relationship_type_by_parent_child($ptype,$type);
            $w->ev(feature_relationship=>[
                                          [subject_id=>$feature_id],
                                          [object_id=>$parent_id],
                                          [type=>$rtype],
                                          [rank=>$rank++],
                                         ]
                  );
        }
    }
    return $feature_id;
}
sub sort_by_strand {
    my $strand = shift || 1;
    my $sfs = shift;
    @$sfs = sort { ($a->start <=> $b->start) * $strand } @$sfs;
    return;
}
sub make_uniquename {
    my $self = shift;
    my $org = shift;
    my $name = shift;
    my $os = $org;
    $os =~ s/\s+/_/g;
    $os =~ s/\(/_/g;
    $os =~ s/\)/_/g;
    $os =~ s/_+/_/g;
    $os =~ s/^_+//g;
    $os =~ s/_+$//g;
    return "$os:$name";
}
sub get_chaos_feature_id {
    my $self = shift;
    my $ob = shift;
    my $id;
    if ($ob->isa("Bio::SeqI")) {
        $id = $ob->accession_number . '.' . ($ob->can('seq_version') ? $ob->seq_version : $ob->version);
    }
    else {
        $ob->isa("Bio::SeqFeatureI") || $self->throw("$ob must be either SeqI or SeqFeatureI");
        if ($ob->primary_id) {
            $id = $ob->primary_id;
        }
        else {
            eval {
                $id = $IDH->generate_unique_persistent_id($ob);
            };
            if ($@) {
                $self->warn($@);
                $id = "$ob"; # last resort - use memory pointer ref
                # will not be persistent, but will be unique
            }
        }
    }
    if (!$id) {
        if ($ob->isa("Bio::SeqFeatureI")) {
            $id = $IDH->generate_unique_persistent_id($ob);
        }
        else {
            $self->throw("Cannot generate a unique persistent ID for a Seq without either primary_id or accession");
        }
    }
    if ($id) {
        $id = $self->context_namespace ? $self->context_namespace . ":" . $id : $id;
    }
    return $id;
}
# interbase and directional semantics
sub bp2ib {
    my $self = shift;
    my $loc = shift;
    my ($s, $e, $str) =
      ref($loc) eq "ARRAY" ? (@$loc) : ($loc->start, $loc->end, $loc->strand);
    $s--;
    if ($str < 0) {
        ($s, $e) = ($e, $s);
    }
    return ($s, $e, $str || 1);
}
sub subpartof {
    my $self = shift;
    my $type = 'partof_'.shift;
    $type =~ s/partof_CDS/CDS_exon/;
    $type =~ s/partof_protein/CDS_exon/;
    $type =~ s/partof_polypeptide/CDS_exon/;
    $type =~ s/partof_\w*RNA/exon/;
    return $type;
}
1;