—————————# $Id: SimilarityPair.pm,v 1.30.4.1 2006/10/02 23:10:28 sendu Exp $
#
# BioPerl module for Bio::SeqFeature::SimilarityPair
#
# Cared for by Hilmar Lapp <hlapp@gmx.net>
#
# Copyright Hilmar Lapp
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::SeqFeature::SimilarityPair - Sequence feature based on the similarity
of two sequences.
=head1 SYNOPSIS
$sim_pair = Bio::SeqFeature::SimilarityPair->from_searchResult($blastHit);
$sim = $sim_pair->query(); # a Bio::SeqFeature::Similarity object - the query
$sim = $sim_pair->hit(); # dto - the hit.
# some properties for the similarity pair
$expect = $sim_pair->significance();
$score = $sim_pair->score();
$bitscore = $sim_pair->bits();
# this will not write the description for the sequence (only its name)
print $sim_pair->query()->gff_string(), "\n";
=head1 DESCRIPTION
Lightweight similarity search result as a pair of Similarity
features. This class inherits off Bio::SeqFeature::FeaturePair and
therefore implements Bio::SeqFeatureI, whereas the two features of the
pair are descendants of Bio::SeqFeature::Generic, with better support
for representing similarity search results in a cleaner way.
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
=head1 AUTHOR - Hilmar Lapp
Email hlapp@gmx.net or hilmar.lapp@pharma.novartis.com
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
# Let the code begin...
package
Bio::SeqFeature::SimilarityPair;
use
strict;
=head2 new
Title : new
Usage : my $similarityPair = new Bio::SeqFeature::SimilarityPair
(-hit => $hit,
-query => $query,
-source => 'blastp');
Function: Initializes a new SimilarityPair object
Returns : Bio::SeqFeature::SimilarityPair
Args : -query => The query in a Feature pair
-hit => (formerly '-subject') the subject/hit in a Feature pair
=cut
sub
new {
my
(
$class
,
@args
) =
@_
;
if
(!
grep
{
lc
(
$_
) eq
"-feature_factory"
; }
@args
) {
# if no overriding factory is provided, provide our preferred one
my
$fact
= Bio::Factory::ObjectFactory->new(
-type
=>
"Bio::SeqFeature::Similarity"
,
-interface
=>
"Bio::SeqFeatureI"
);
push
(
@args
,
'-feature_factory'
,
$fact
);
}
my
$self
=
$class
->SUPER::new(
@args
);
my
(
$primary
,
$hit
,
$query
,
$fea1
,
$source
,
$sbjct
) =
$self
->_rearrange([
qw(PRIMARY
HIT
QUERY
FEATURE1
SOURCE
SUBJECT
)
],
@args
);
if
(
$sbjct
) {
# undeprecated by Jason before 1.1 release
# $self->deprecated("use of -subject deprecated: SimilarityPair now uses 'hit'");
if
(!
$hit
) {
$hit
=
$sbjct
}
else
{
$self
->
warn
(
"-hit and -subject were specified, using -hit and ignoring -subject"
);
}
}
# set the query and subject feature if provided
$self
->query(
$query
)
if
$query
&& !
$fea1
;
$hit
&&
$self
->hit(
$hit
);
# the following refer to feature1, which is guaranteed to exist
if
(
defined
$primary
|| !
defined
$self
->primary_tag) {
$primary
=
'similarity'
unless
defined
$primary
;
$self
->primary_tag(
$primary
);
}
$source
&&
$self
->source_tag(
$source
);
return
$self
;
}
#
# Everything else is just inherited from SeqFeature::FeaturePair.
#
=head2 query
Title : query
Usage : $query_feature = $obj->query();
$obj->query($query_feature);
Function: The query object for this similarity pair
Returns : Bio::SeqFeature::Similarity
Args : [optional] Bio::SeqFeature::Similarity
See L<Bio::SeqFeature::Similarity>, L<Bio::SeqFeature::FeaturePair>
=cut
sub
query {
return
shift
->feature1(
@_
);
}
=head2 subject
Title : subject
Usage : $sbjct_feature = $obj->subject();
$obj->subject($sbjct_feature);
Function: Get/Set Subject for a SimilarityPair
Returns : Bio::SeqFeature::Similarity
Args : [optional] Bio::SeqFeature::Similarity
Notes : Deprecated. Use the method 'hit' instead
=cut
sub
subject {
my
$self
=
shift
;
# $self->deprecated("Method subject deprecated: use hit() instead");
$self
->hit(
@_
);
}
=head2 hit
Title : hit
Usage : $sbjct_feature = $obj->hit();
$obj->hit($sbjct_feature);
Function: Get/Set Hit for a SimilarityPair
Returns : Bio::SeqFeature::Similarity
Args : [optional] Bio::SeqFeature::Similarity
=cut
sub
hit {
return
shift
->feature2(
@_
);
}
=head2 source_tag
Title : source_tag
Usage : $source = $obj->source_tag(); # i.e., program
$obj->source_tag($evalue);
Function: Gets the source tag (program name typically) for a feature
Returns : string
Args : [optional] string
=cut
sub
source_tag {
my
(
$self
,
@args
) =
@_
;
if
(
@args
) {
$self
->hit()->source_tag(
@args
);
}
return
$self
->query()->source_tag(
@args
);
}
=head2 significance
Title : significance
Usage : $evalue = $obj->significance();
$obj->significance($evalue);
Function:
Returns :
Args :
=cut
sub
significance {
my
(
$self
,
@args
) =
@_
;
if
(
@args
) {
$self
->hit()->significance(
@args
);
}
return
$self
->query()->significance(
@args
);
}
=head2 score
Title : score
Usage : $score = $obj->score();
$obj->score($value);
Function:
Returns :
Args :
=cut
sub
score {
my
(
$self
,
@args
) =
@_
;
if
(
@args
) {
$self
->hit()->score(
@args
);
}
# Note: You might think it's only getting set on the hit object.
# Actually, it's getting set on both hit and query.
return
$self
->query()->score(
@args
);
}
=head2 bits
Title : bits
Usage : $bits = $obj->bits();
$obj->bits($value);
Function:
Returns :
Args :
=cut
sub
bits {
my
(
$self
,
@args
) =
@_
;
if
(
@args
) {
$self
->hit()->bits(
@args
);
}
return
$self
->query()->bits(
@args
);
}
#################################################################
# aliases for backwards compatibility or convenience #
#################################################################
*sbjct
= \
&subject
;
1;