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# $Id: Singlet.pm,v 1.9.4.1 2006/10/02 23:10:12 sendu Exp $
#
# BioPerl module for Bio::Assembly::Singlet
#
# Cared for by Chad Matsalla <bioinformatics1 at dieselwurks.com>
#
# Copyright Chad Matsalla
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::Assembly::Singlet - Perl module to hold and manipulate
singlets from sequence assembly contigs.
=head1 SYNOPSIS
# Module loading
use Bio::Assembly::IO;
# Assembly loading methods
$aio = new Bio::Assembly::IO(-file=>"test.ace.1",
-format=>'phrap');
$assembly = $aio->next_assembly;
foreach $singlet ($assembly->all_singlets) {
# do something
}
=head1 DESCRIPTION
A singlet is a sequence that phrap was unable to align to any other sequences.
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to the
Bioperl mailing lists Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
=head1 AUTHOR - Chad S. Matsalla
bioinformatics1 at dieselwurks.com
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
#'
use strict;
use Dumpvalue();
my $dumper = new Dumpvalue();
$dumper->veryCompact(1);
use base qw(Bio::Assembly::Contig Bio::Root::Root Bio::Align::AlignI Bio::Assembly::Contig);
sub new {
my ($class,%ARG) = @_;
my $self = $class->SUPER::new(%ARG);
my $args = \%ARG;
bless ($self,$class);
if ($args->{'-seq'}) {
$self->seq_to_singlet($args->{'-seq'});
}
return $self;
}
=head2 seq_to_singlet
Title : seq_to_singlet
Usage : my $singlet = $io->seq_to_singlet($seq)
Function: Wrap the information for a singlet as a Bio::Assembly::Singlet
Returns : A Bio::Assembly::Singlet object
Args : A Bio::Seq-compliant object
=cut
sub seq_to_singlet {
my ($self,$seq) = @_;
$self->seqref($seq);
$self->strand(1);
my $lseq = new Bio::LocatableSeq(
-seq => $seq->seq(),
-start => 1,
-end => $seq->length(),
-id => $seq->display_id());
$lseq->{chromatfilename} = $seq->{'chromatfilename'};
$lseq->{phdfilename} = $seq->{'phdfilename'};
$self->set_consensus_sequence($lseq);
if (UNIVERSAL::isa($seq,"Bio::Seq::Quality")) {
$self->set_consensus_quality($seq)
}
else {
# print("seq_to_singlet: the sequence (".$seq->desc().") is not a Bio::Seq::quality. it is this ($seq)\n");
}
$self->add_seq($lseq);
}
=head2 id
Title : id
Usage : my $id = $singlet->id('chad matsalla')
Function:
Returns :
Args :
=cut
sub id {
my $self = shift;
# print("Getting the id for this thing:\n");
# $dumper->dumpValue($self->seqref());
# print("This is the id: (".$self->seqref()->id().")\n");
return $self->seqref()->id();
}
=head2 seqref
Title : seqref
Usage : my $seqref = $singlet->seqref($seq);
Function: Set the sequence to which this Singlet refers
Returns : A Bio::Seq-compliant object
Args :
=cut
sub seqref {
my ($self,$seq) = @_;
if ($seq) { $self->{'seqref'} = $seq; }
return $self->{'seqref'};
}
=head2 chromatfilename
Title : chromatfilename
Usage : my $chromatfilename = $singlet->chromatfilename($newfilename);
Function: Get the name of the chromatfile for this singlet
Returns : A string.
Args : If a string is provided, the chromatfilename will be set to that value.
=cut
sub chromatfilename {
my ($self,$name) = @_;
if ($name) { $self->{'chromatfilename'} = $name; }
return $self->{'chromatfilename'};
}
=head2 phdfilename
Title : phdfilename
Usage : my $phdfilename = $singlet->phdfilename($newfilename);
Function: Get the name of the phdfile for this singlet
Returns : A string.
Args : If a string is provided, the phdfilename will be set to that value.
=cut
sub phdfilename {
my ($self,$name) = @_;
if ($name) { $self->{phdfilename} = $name; }
return $self->{'phdfilename'};
}
1;