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=pod
=head1 NAME
Bio::FeatureIO::ptt - read/write features in PTT format
=head1 SYNOPSIS
# read features
my $fin = Bio::FeatureIO->new(-file=>'genes.ptt', -format=>'ptt');
my @cds;
while (my $f = $fin->next_feature) {
push @cds, $f if $f->strand > 0;
}
# write features (NOT IMPLEMENTED)
my $fout = Bio::FeatureIO->new(-fh=>\*STDOUT, -format=>'ptt');
for my $f (@cds) {
$fout->write_feature($f);
}
=head1 DESCRIPTION
The PTT file format is a table of protein features.
It is used mainly by NCBI who produce PTT files for
all their published genomes found in L<ftp://ftp.ncbi.nih.gov/genomes/>.
It has the following format:
==over 4
=item Line 1
Description of sequence to which the features belong
eg. "Leptospira interrogans chromosome II, complete sequence - 0..358943"
It is usually equivalent to the DEFINITION line of a Genbank file,
with the length of the sequence appended. It is unclear why "0" is
used as a starting range, it should be "1".
=item Line 2
Number of feature lines in the table
eg. "367 proteins"
=item Line 3
Column headers, tab separated
eg. "Location Strand Length PID Gene Synonym Code COG Product"
Location : "begin..end" span of feature
Strand : "+" or "-"
Length : number of amino acids excluding the stop codon
PID : analogous to Genbank /db_xref="GI:xxxxxxxxx"
Gene : analogous to Genbank /gene="xxxx"
Synonym : analogous to Genbank /locus_tag="xxxx"
Synonym : analogous to Genbank /locus_tag="xxxx"
COG : CDD COG code with COG letter categories appended
Product : analogous to Genbank /product="xxxx"
=item Line 4 onwards
Feature lines, nine columns, tab separated, "-" used for empty fields
eg. "2491..3423 + 310 24217063 metF LB002 - COG0685E 5,10-methylenetetrahydrofolate reductase"
==back
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via
the web:
=head1 AUTHOR - Torsten Seemann
Email torsten.seemann AT infotech.monash.edu.au
=head1 CONTRIBUTORS
Based on bed.pm and gff.pm by Allen Day.
=head1 APPENDIX
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
=cut
# Let the code begin...
require 5.006_001;
use strict;
# map tab-separated column number to field name
our %NAME_OF = (
0 => 'Location',
1 => 'Strand',
2 => 'Length',
3 => 'PID',
4 => 'Gene',
5 => 'Synonym',
6 => 'Code',
7 => 'COG',
8 => 'Product',
);
our $NUM_COL = 9;
=head2 _initialize
Title : _initialize
Function: Reading? parses the header of the input
Writing?
=cut
sub _initialize {
my($self,%arg) = @_;
$self->SUPER::_initialize(%arg);
if ($self->mode eq 'r') {
# Line 1
my $desc = $self->_readline();
chomp $desc;
$self->description($desc);
# Line 2
my $line = $self->_readline();
$line =~ m/^(\d+) proteins/ or $self->throw("Invalid protein count");
$self->protein_count($1);
# Line 3
$self->_readline();
}
}
=head2 next_feature
Title : next_feature
Usage : $io->next_feature()
Function: read the next feature from the PTT file
Example :
Args :
Returns : Bio::SeqFeatureI object
=cut
sub next_feature {
my $self = shift;
$self->mode eq 'r' || return; # returns if can't read next_feature when we're in write mode
my $line = $self->_readline() or return; # returns if end of file, no more features?
chomp $line;
my @col = split m/\t/, $line;
@col==$NUM_COL or $self->throw("Too many columns for PTT line");
$col[0] =~ m/(\d+)\.\.(\d+)/ or $self->throw("Invalid location (column 1)");
my $feat = Bio::SeqFeature::Generic->new(-start=>$1, -end=>$2, -primary=>'CDS');
$col[1] =~ m/^([+-])$/ or $self->throw("Invalid strand (column 2)");
$feat->strand($1 eq '+' ? +1 : -1);
for my $i (2 .. $NUM_COL-1) {
$feat->add_tag_value($NAME_OF{$i}, $col[$i]) if $col[$i] ne '-';
}
return $feat;
}
=head2 write_feature (NOT IMPLEMENTED)
Title : write_feature
Usage : $io->write_feature($feature)
Function: write a Bio::SeqFeatureI object in PTT format
Example :
Args : Bio::SeqFeatureI object
Returns :
=cut
sub write_feature {
shift->throw_not_implemented;
}
=head2 description
Title : description
Usage : $obj->description($newval)
Function: set/get the PTT file description for/from line one
Example :
Returns : value of description (a scalar)
Args : on set, new value (a scalar or undef, optional)
=cut
sub description {
my $self = shift;
return $self->{'description'} = shift if @_;
return $self->{'description'};
}
=head2 protein_count
Title : protein_count
Usage : $obj->protein_count($newval)
Function: set/get the PTT protein count for/from line two
Example :
Args : on set, new value (a scalar or undef, optional)
Returns : value of protein_count (a scalar)
=cut
sub protein_count {
my $self = shift;
return $self->{'protein_count'} = shift if @_;
return $self->{'protein_count'};
}
1;