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# Copyright Balamurugan Kumarasamy
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::Tools::Run::Prints
=head1 SYNOPSIS
Build a Prints factory
my @params = ('DB',$dbfile);
my $factory = Bio::Tools::Run::Prints->new($params);
# Pass the factory a Bio::Seq object
# @feats is an array of Bio::SeqFeature::Generic objects
my @feats = $factory->run($seq);
=head1 DESCRIPTION
FingerPRINTScan II is a PRINTS fingerprint identification algorithm.
Copyright (C) 1998,1999 Phil Scordis
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
=head1 AUTHOR - Bala
Email savikalpa@fugu-sg.org
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
use vars qw($AUTOLOAD @ISA $PROGRAM $PROGRAMDIR
$PROGRAMNAME @PRINTS_PARAMS
%OK_FIELD);
use strict;
@ISA = qw(Bio::Root::Root Bio::Tools::Run::WrapperBase);
BEGIN {
@PRINTS_PARAMS=qw(DB PROGRAM VERBOSE);
foreach my $attr ( @PRINTS_PARAMS)
{ $OK_FIELD{$attr}++; }
}
=head2 program_name
Title : program_name
Usage : $factory>program_name()
Function: holds the program name
Returns: string
Args : None
=cut
sub program_name {
return 'FingerPRINTScan';
}
=head2 program_dir
Title : program_dir
Usage : $factory->program_dir(@params)
Function: returns the program directory, obtiained from ENV variable.
Returns: string
Args :
=cut
sub program_dir {
return Bio::Root::IO->catfile($ENV{PRINTSDIR}) if $ENV{PRINTSDIR};
}
sub AUTOLOAD {
my $self = shift;
my $attr = $AUTOLOAD;
$attr =~ s/.*:://;
$attr = uc $attr;
$self->throw("Unallowed parameter: $attr !") unless $OK_FIELD{$attr};
$self->{$attr} = shift if @_;
return $self->{$attr};
}
=head2 new
Title : new
Usage : $prints->new(@params)
Function: creates a new Prints factory
Returns: Bio::Tools::Run::Prints
Args :
=cut
sub new {
my ($class,@args) = @_;
my $self = $class->SUPER::new(@args);
$self->io->_initialize_io();
my ($attr, $value);
while (@args) {
$attr = shift @args;
$value = shift @args;
next if( $attr =~ /^-/ ); # don't want named parameters
if ($attr =~/PROGRAM/i) {
$self->executable($value);
next;
}
$self->$attr($value);
}
return $self;
}
=head2 predict_protein_features
Title : predict_protein_features()
Usage : DEPRECATED. Use $obj->run($seqFile) instead.
Function: Runs Prints and creates an array of featrues
Returns : An array of Bio::SeqFeature::Generic objects
Args : A Bio::PrimarySeqI
=cut
sub predict_protein_features{
return shift->run(@_);
}
=head2 run
Title : run
Usage : $obj->run($seq)
Function: Runs Prints
Returns : An array of Bio::SeqFeature::Generic objects
Args : A Bio::PrimarySeqI, or a Fasta file name
=cut
sub run{
my ($self,$seq) = @_;
my @feats;
if (ref($seq) ){# it is an object
if (ref($seq) =~ /GLOB/) {
$self->throw("cannot use filehandle");
}
my $infile1 = $self->_writeSeqFile($seq);
$self->_input($infile1);
@feats = $self->_run();
unlink $infile1;
}
else {
#The clone object is not a seq object but a file.
#Perhaps should check here or before if this file is fasta format...if not die
#Here the file does not need to be created or deleted. Its already written and may be used by other runnables.
$self->_input($seq);
@feats = $self->_run();
}
return @feats;
}
=head2 _input
Title : _input
Usage : obj->_input($seqFile)
Function: Internal(not to be used directly)
Returns :
Args :
=cut
sub _input() {
my ($self,$infile1) = @_;
if(defined $infile1){
$self->{'input'}=$infile1;
}
return $self->{'input'};
}
=head2 _run
Title : _run
Usage : $obj->_run()
Function: Internal(not to be used directly)
Returns : An array of Bio::SeqFeature::Generic objects
Args :
=cut
sub _run {
my ($self)= @_;
my ($tfh,$outfile) = $self->io->tempfile(-dir=>$self->tempdir());
close($tfh);
undef $tfh;
my $str =$self->executable." ".$self->DB." ".$self->_input." -fjR >".$outfile;
my $status = system($str);
$self->throw( "Prints call ($str) crashed: $? \n") unless $status==0;
my $filehandle;
if (ref ($outfile) !~ /GLOB/) {
open (PRINTS, "<".$outfile) or $self->throw ("Couldn't open file ".$outfile.": $!\n");
$filehandle = \*PRINTS;
}
else {
$filehandle = $outfile;
}
my $prints_parser = Bio::Tools::Prints->new(-fh=>$filehandle);
my @prints_feat;
while(my $prints_feat = $prints_parser->next_result){
push @prints_feat, $prints_feat;
}
$self->cleanup();
unlink $outfile;
return @prints_feat;
}
=head2 _writeSeqFile
Title : _writeSeqFile
Usage : obj->_writeSeqFile($seq)
Function: Internal(not to be used directly)
Returns :
Args :
=cut
sub _writeSeqFile{
my ($self,$seq) = @_;
my ($tfh,$inputfile) = $self->io->tempfile(-dir=>$self->tempdir());
my $in = Bio::SeqIO->new(-fh => $tfh , '-format' => 'Fasta');
$in->write_seq($seq);
$in->close();
undef $in;
close($tfh);
undef $tfh;
return $inputfile;
}
1;