NAME
Bio::Tools::Run::PiseApplication::tacg
SYNOPSIS
#DESCRIPTION
Bio::Tools::Run::PiseApplication::tacg
Bioperl class for:
TACG Restriction Enzyme analysis (Mangalam)
Parameters:
(see also:
http://bioweb.pasteur.fr/seqanal/interfaces/tacg.html
for available values):
tacg (String)
sequence (Sequence)
DNA Sequence (raw sequence)
beginning (Integer)
Beginning of a subsequence in your sequence (-b)
end (Integer)
End of a subsequence in your sequence (-e)
topology (Excl)
Form (or topology) of DNA (-f)
degeneracy (Excl)
Degeneracy flag - controls input and analysis of degenerate sequence input (-D)
codon (Excl)
Codon Usage table to use for translation (-C)
order_by_cut (Switch)
Order the output by number of cuts/fragments (-c)
width (Integer)
Output width (between 60 and 210) (-w)
graphic (Excl)
Histogram output (-G)
binsize (Integer)
Step size in histogram
enzymes (String)
Enzymes selection list (separated by comma)
max_cut (Integer)
Maximum number of cuts allowed in sequence (-M)
min_cut (Integer)
Minimum number of cuts in sequence for the enzyme to be selected (-m)
magnitude (Excl)
Select enzymes by magnitude of recognition site (-n)
overhang (Excl)
Select enzymes by overhang generated (-o)
summary (Switch)
Summary of site information (-s)
print_fragments (Excl)
Table of fragments (-F)
sites (Switch)
Table of actual cut sites (a la Strider) (-S)
ladder_map (Switch)
Ladder map of selected enzymes (-l)
gel_map (Switch)
Pseudo-graphic gel map (-g)
cutoff (Integer)
Low-end cutoff in number of bases for gel map (>= 10)
linear_map (Switch)
Linear map a la Strider (-L)
translation (Switch)
Linear co-translation (-t,-T)
translation_frames (Excl)
Translation in how many frames
three_letter (Switch)
Translation in 3-letter code (-T)
orf (Switch)
Do an ORF analysis
frame (List)
Frames to search
min_size (Integer)
Min ORF size
pattern_search (Switch)
Do a pattern search (-p)
pattern (String)
Pattern (<30 IUPAC character)
errors (Integer)
Max number of errors that are tolerated (<6) (-p)
name (String)
Label of pattern
proximity (Switch)
Do a proximity search
distance (String)
Distance between factors
nameA (String)
Name of first factor (nameA)
nameB (String)
Name of second factor (nameB)
quiet (String)FEEDBACK
Mailing Lists
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/MailList.shtml - About the mailing listsReporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via email or the web:
bioperl-bugs@bioperl.org
http://bioperl.org/bioperl-bugs/AUTHOR
Catherine Letondal (letondal@pasteur.fr)
COPYRIGHT
Copyright (C) 2003 Institut Pasteur & Catherine Letondal. All Rights Reserved.
This module is free software; you can redistribute it and/or modify it under the same terms as Perl itself.
DISCLAIMER
This software is provided "as is" without warranty of any kind.
SEE ALSO
http://bioweb.pasteur.fr/seqanal/interfaces/tacg.html
Bio::Tools::Run::PiseApplication
Bio::Tools::Run::AnalysisFactory::Pise
Bio::Tools::Run::PiseJob
new
Title : new()
Usage : my $tacg = Bio::Tools::Run::PiseApplication::tacg->new($location, $email, @params);
Function: Creates a Bio::Tools::Run::PiseApplication::tacg object.
This method should not be used directly, but rather by
a Bio::Tools::Run::AnalysisFactory::Pise instance.
my $factory = Bio::Tools::Run::AnalysisFactory::Pise->new();
my $tacg = $factory->program('tacg');
Example : -
Returns : An instance of Bio::Tools::Run::PiseApplication::tacg.