NAME

Bio::Grep::Backend::Hypa - HyPa back-end

SYNOPSIS

  use Bio::Grep::Backend::Hypa;
 
 
  # construct the Hypa back-end 
  my $sbe = Bio::Grep::Backend::Hypa->new();
 
  $sbe->settings->tmppath('tmp');
  $sbe->settings->datapath('data');
  
  # generate a Hypa suffix array. you have to do this only once.
  $sbe->generate_database('ATH1.cdna', 'AGI Transcripts (- introns, + UTRs)');
 
  my %local_dbs_description = $sbe->get_databases();
  my @local_dbs = sort keys %local_dbs_description;
 
  # take first available database in our test
  $sbe->settings->database($local_dbs[0]);
 
  my $seq = 'UGAACAGAAAGCUCAUGAGCC'; 
 
  # search for the reverse complement and allow 4 mismatches
  # display 5 bases upstream and downstream of the match
  $sbe->settings->query($seq);
  $sbe->settings->reverse_complement(1);
  $sbe->settings->mismatches(4);
  $sbe->settings->upstream(5);
  $sbe->settings->downstream(5);
  
  # hypa does not generate alignments, but EMBOSS will automatically calculate some for
  # you. If things should be fast, turn this off        
  # $sbe->settings->no_alignments(1);
 
  # with the features hash, you can check if the back-end supports
  # a special feature. Allow wobble pairs (GU counts 0 or 0.5)  
  if (defined($sbe->features->{GUMISMATCHES})) {
          $sbe->settings->gumismatches(0.5);            
  } else {
     print "\nBack-end does not support wobble pairs\n";
  }     
 
  $sbe->search();
 
  # output all informations we have!
  while ( my $res = $sbe->next_res ) {
     print $res->sequence->id . "\n";
     print $res->mark_subject_uppercase() . "\n";
     print $res->alignment_string() . "\n\n";
  }
   

DESCRIPTION

Bio::Grep::Backend::Hypa searches for a query in a Hypa suffix array.

NOTE 1: Hypa can not calculate alignments. But because we have the exact position of the match, the alignment calculation shouldn't be too slow (In agrep, we align query and sequence, here query and approximate match).

NOTE 2: online is available. But it is not recommended.

METHODS

See Bio::Grep::Backend::BackendI for other methods.

Bio::Grep::Backend::Hypa->new()

This function constructs a Hypa back-end object

   my $sbe = Bio::Grep::Backend::Hypa->new();

$sbe->available_sort_modes()

Returns all available sort modes as hash. keys are sort modes, values a short description.

   $sbe->sort('ga');

Available sortmodes in Hypa:

            ga  : 'ascending order of dG'
            gd  : 'descending order of dG'

Note that 'ga' and 'gd' require that search results have dG set. Bio::Grep::RNA ships with filters for free energy calculation. Also note that these two sort options require that we load all results in memory.

BUGS AND LIMITATIONS

Hypa currently does not return the correct downstream and upstream regions if the requested region is larger then available (if hit is near 5' or 3' end).

Hypa does not generate alignments so we use EMBOSS for that. This is slow and produces a lot of IO.

Please report any bugs or feature requests to bug-bio-grep@rt.cpan.org, or through the web interface at http://rt.cpan.org.

SEE ALSO

Bio::Grep::Backend::BackendI Bio::Grep::SearchSettings Bio::SeqIO

AUTHOR

Markus Riester, <mriester@gmx.de>

LICENCE AND COPYRIGHT

Based on Weigel::Search v0.13

Copyright (C) 2005-2006 by Max Planck Institute for Developmental Biology, Tuebingen.

This module is free software; you can redistribute it and/or modify it under the same terms as Perl itself.

DISCLAIMER OF WARRANTY

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