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Christopher Fields
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Bio::SeqI - [Developers] Abstract Interface of Sequence (with features)


    # Bio::SeqI is the interface class for sequences.

    # If you are a newcomer to bioperl, you should
    # start with Bio::Seq documentation. This
    # documentation is mainly for developers using
    # Bioperl.

    # Bio::SeqI implements Bio::PrimarySeqI
    $seq      = $seqobj->seq(); # actual sequence as a string
    $seqstr   = $seqobj->subseq(10,50);

    # Bio::SeqI has annotationcollections

    $ann      = $seqobj->annotation(); # annotation object

    # Bio::SeqI has sequence features
    # features must implement Bio::SeqFeatureI

    @features = $seqobj->get_SeqFeatures();     # just top level
    @features = $seqobj->get_all_SeqFeatures(); # descend into sub features


Bio::SeqI is the abstract interface of annotated Sequences. These methods are those which you can be guaranteed to get for any Bio::SeqI. For most users of the package the documentation (and methods) in this class are not at useful - this is a developers only class which defines what methods have to be implemented by other Perl objects to comply to the Bio::SeqI interface. Go "perldoc Bio::Seq" or "man Bio::Seq" for more information.

There aren't many method here, because too many complicated functions here would prevent implementations which are just wrappers around a database or similar delayed mechanisms.

Most of the clever stuff happens inside the SeqFeatureI system.

A good reference implementation is Bio::Seq which is a pure perl implementation of this class with a lot of extra pieces for extra manipulation. However, if you want to be able to use any sequence object in your analysis, if you can do it just using these methods, then you know you will be future proof and compatible with other implementations of Seq.


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rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

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AUTHOR - Ewan Birney

Email birney@ebi.ac.uk


The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _


 Title   : get_SeqFeatures
 Usage   : my @feats = $seq->get_SeqFeatures();
 Function: retrieve just the toplevel sequence features attached to this seq
 Returns : array of Bio::SeqFeatureI objects
 Args    : none

This method comes through extension of Bio::FeatureHolderI. See Bio::FeatureHolderI and Bio::SeqFeatureI for more information.


 Title   : get_all_SeqFeatures
 Usage   : my @feats = $seq->get_all_SeqFeatures();
 Function: returns all SeqFeatures, including sub SeqFeatures
 Returns : an array of Bio::SeqFeatureI objects
 Args    : none

This method comes through extension of Bio::FeatureHolderI. See Bio::FeatureHolderI and Bio::SeqFeatureI for more information.


 Title   : feature_count
 Usage   : my $count = $seq->feature_count();
 Function: Return the number of SeqFeatures attached to a sequence
 Returns : integer representing the number of SeqFeatures
 Args    : none

This method comes through extension of Bio::FeatureHolderI. See Bio::FeatureHolderI for more information.


 Title   : seq
 Usage   : my $string = $seq->seq();
 Function: Retrieves the sequence string for the sequence object
 Returns : string
 Args    : none


 Title   : write_GFF
 Usage   : $seq->write_GFF(\*FILEHANDLE);
 Function: Convenience method to write out all the sequence features
           in GFF format to the provided filehandle (STDOUT by default)
 Returns : none
 Args    : [optional] filehandle to write to (default is STDOUT)


 Title   : annotation
 Usage   : my $ann = $seq->annotation($seq_obj);
 Function: retrieve the attached annotation object
 Returns : Bio::AnnotationCollectionI or none;

See Bio::AnnotationCollectionI and Bio::Annotation::Collection for more information. This method comes through extension from Bio::AnnotatableI.


 Title   : species
 Usage   :
 Function: Gets or sets the species
 Example : my $species = $seq->species();
 Returns : Bio::Species object
 Args    : Bio::Species object or none;

See Bio::Species for more information


 Title   : primary_seq
 Usage   : my $primaryseq = $seq->primary_seq($newval)
 Function: Retrieve the underlying Bio::PrimarySeqI object if available.
           This is in the event one has a sequence with lots of features
           but want to be able to narrow the object to just one with
           the basics of a sequence (no features or annotations).
 Returns : Bio::PrimarySeqI
 Args    : Bio::PrimarySeqI or none;

See Bio::PrimarySeqI for more information